tetano
Editor, Senior Moderator
Nat Commun
. 2020 Nov 11;11(1):5703.
doi: 10.1038/s41467-020-19545-8.
Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19
Shidan Tosif[SUP] 1 2 3 [/SUP], Melanie R Neeland[SUP] 4 5 [/SUP], Philip Sutton[SUP] 4 5 [/SUP], Paul V Licciardi[SUP] 4 5 [/SUP], Sohinee Sarkar[SUP] 4 5 [/SUP], Kevin J Selva[SUP] 6 [/SUP], Lien Anh Ha Do[SUP] 4 5 [/SUP], Celeste Donato[SUP] 4 5 [/SUP], Zheng Quan Toh[SUP] 4 5 [/SUP], Rachel Higgins[SUP] 5 [/SUP], Carolien Van de Sandt[SUP] 6 7 [/SUP], Melissa M Lemke[SUP] 8 [/SUP], Christina Y Lee[SUP] 8 [/SUP], Suzanne K Shoffner[SUP] 8 [/SUP], Katie L Flanagan[SUP] 9 10 11 12 [/SUP], Kelly B Arnold[SUP] 8 [/SUP], Francesca L Mordant[SUP] 6 [/SUP], Kim Mulholland[SUP] 4 5 13 [/SUP], Julie Bines[SUP] 4 5 14 [/SUP], Kate Dohle[SUP] 5 [/SUP], Daniel G Pellicci[SUP] 4 5 [/SUP], Nigel Curtis[SUP] 4 5 13 [/SUP], Sarah McNab[SUP] 4 5 15 [/SUP], Andrew Steer[SUP] 4 5 13 [/SUP], Richard Saffery[SUP] 4 5 [/SUP], Kanta Subbarao[SUP] 6 16 [/SUP], Amy W Chung[SUP] 6 [/SUP], Katherine Kedzierska[SUP] 6 [/SUP], David P Burgner[SUP] 4 5 13 [/SUP], Nigel W Crawford[SUP] 4 5 15 [/SUP]
Affiliations
Abstract
Compared to adults, children with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have predominantly mild or asymptomatic infections, but the underlying immunological differences remain unclear. Here, we describe clinical features, virology, longitudinal cellular, and cytokine immune profile, SARS-CoV-2-specific serology and salivary antibody responses in a family of two parents with PCR-confirmed symptomatic SARS-CoV-2 infection and their three children, who tested repeatedly SARS-CoV-2 PCR negative. Cellular immune profiles and cytokine responses of all children are similar to their parents at all timepoints. All family members have salivary anti-SARS-CoV-2 antibodies detected, predominantly IgA, that coincide with symptom resolution in 3 of 4 symptomatic members. Plasma from both parents and one child have IgG antibody against the S1 protein and virus-neutralizing activity detected. Using a systems serology approach, we demonstrate higher levels of SARS-CoV-2-specific antibody features of these family members compared to healthy controls. These data indicate that children can mount an immune response to SARS-CoV-2 without virological confirmation of infection, raising the possibility that immunity in children can prevent the establishment of SARS-CoV-2 infection. Relying on routine virological and serological testing may not identify exposed children, with implications for epidemiological and clinical studies across the life-span.
. 2020 Nov 11;11(1):5703.
doi: 10.1038/s41467-020-19545-8.
Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19
Shidan Tosif[SUP] 1 2 3 [/SUP], Melanie R Neeland[SUP] 4 5 [/SUP], Philip Sutton[SUP] 4 5 [/SUP], Paul V Licciardi[SUP] 4 5 [/SUP], Sohinee Sarkar[SUP] 4 5 [/SUP], Kevin J Selva[SUP] 6 [/SUP], Lien Anh Ha Do[SUP] 4 5 [/SUP], Celeste Donato[SUP] 4 5 [/SUP], Zheng Quan Toh[SUP] 4 5 [/SUP], Rachel Higgins[SUP] 5 [/SUP], Carolien Van de Sandt[SUP] 6 7 [/SUP], Melissa M Lemke[SUP] 8 [/SUP], Christina Y Lee[SUP] 8 [/SUP], Suzanne K Shoffner[SUP] 8 [/SUP], Katie L Flanagan[SUP] 9 10 11 12 [/SUP], Kelly B Arnold[SUP] 8 [/SUP], Francesca L Mordant[SUP] 6 [/SUP], Kim Mulholland[SUP] 4 5 13 [/SUP], Julie Bines[SUP] 4 5 14 [/SUP], Kate Dohle[SUP] 5 [/SUP], Daniel G Pellicci[SUP] 4 5 [/SUP], Nigel Curtis[SUP] 4 5 13 [/SUP], Sarah McNab[SUP] 4 5 15 [/SUP], Andrew Steer[SUP] 4 5 13 [/SUP], Richard Saffery[SUP] 4 5 [/SUP], Kanta Subbarao[SUP] 6 16 [/SUP], Amy W Chung[SUP] 6 [/SUP], Katherine Kedzierska[SUP] 6 [/SUP], David P Burgner[SUP] 4 5 13 [/SUP], Nigel W Crawford[SUP] 4 5 15 [/SUP]
Affiliations
- PMID: 33177504
- DOI: 10.1038/s41467-020-19545-8
Abstract
Compared to adults, children with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have predominantly mild or asymptomatic infections, but the underlying immunological differences remain unclear. Here, we describe clinical features, virology, longitudinal cellular, and cytokine immune profile, SARS-CoV-2-specific serology and salivary antibody responses in a family of two parents with PCR-confirmed symptomatic SARS-CoV-2 infection and their three children, who tested repeatedly SARS-CoV-2 PCR negative. Cellular immune profiles and cytokine responses of all children are similar to their parents at all timepoints. All family members have salivary anti-SARS-CoV-2 antibodies detected, predominantly IgA, that coincide with symptom resolution in 3 of 4 symptomatic members. Plasma from both parents and one child have IgG antibody against the S1 protein and virus-neutralizing activity detected. Using a systems serology approach, we demonstrate higher levels of SARS-CoV-2-specific antibody features of these family members compared to healthy controls. These data indicate that children can mount an immune response to SARS-CoV-2 without virological confirmation of infection, raising the possibility that immunity in children can prevent the establishment of SARS-CoV-2 infection. Relying on routine virological and serological testing may not identify exposed children, with implications for epidemiological and clinical studies across the life-span.