tetano
Editor, Senior Moderator
Nat Commun
. 2024 Feb 5;15(1):1048.
doi: 10.1038/s41467-024-45453-2. Isolation and characterization of a pangolin-borne HKU4-related coronavirus that potentially infects human-DPP4-transgenic mice
Luo-Yuan Xia[SUP] #[/SUP][SUP] 1 [/SUP], Zhen-Fei Wang[SUP] #[/SUP][SUP] 1 2 [/SUP], Xiao-Ming Cui[SUP] #[/SUP][SUP] 3 4 [/SUP], Yuan-Guo Li[SUP] #[/SUP][SUP] 2 [/SUP], Run-Ze Ye[SUP] #[/SUP][SUP] 1 [/SUP], Dai-Yun Zhu[SUP] 3 [/SUP], Fang-Xu Li[SUP] 2 [/SUP], Jie Zhang[SUP] 3 [/SUP], Wen-Hao Wang[SUP] 2 [/SUP], Ming-Zhu Zhang[SUP] 1 [/SUP], Wan-Ying Gao[SUP] 1 [/SUP], Lian-Feng Li[SUP] 1 [/SUP], Teng-Cheng Que[SUP] 5 [/SUP], Tie-Cheng Wang[SUP] 2 [/SUP], Na Jia[SUP] 3 4 [/SUP], Jia-Fu Jiang[SUP] 6 7 [/SUP], Yu-Wei Gao[SUP] 8 [/SUP], Wu-Chun Cao[SUP] 9 10 11 [/SUP]
Affiliations
We recently detected a HKU4-related coronavirus in subgenus Merbecovirus (named pangolin-CoV-HKU4-P251T) from a Malayan pangolin[SUP]1[/SUP]. Here we report isolation and characterization of pangolin-CoV-HKU4-P251T, the genome sequence of which is closest to that of a coronavirus from the greater bamboo bat (Tylonycteris robustula) in Yunnan Province, China, with a 94.3% nucleotide identity. Pangolin-CoV-HKU4-P251T is able to infect human cell lines, and replicates more efficiently in cells that express human-dipeptidyl-peptidase-4 (hDPP4)-expressing and pangolin-DPP4-expressing cells than in bat-DPP4-expressing cells. After intranasal inoculation with pangolin-CoV-HKU4-P251, hDPP4-transgenic female mice are likely infected, showing persistent viral RNA copy numbers in the lungs. Progressive interstitial pneumonia developed in the infected mice, characterized by the accumulation of macrophages, and increase of antiviral cytokines, proinflammatory cytokines, and chemokines in lung tissues. These findings suggest that the pangolin-borne HKU4-related coronavirus has a potential for emerging as a human pathogen by using hDPP4.
. 2024 Feb 5;15(1):1048.
doi: 10.1038/s41467-024-45453-2. Isolation and characterization of a pangolin-borne HKU4-related coronavirus that potentially infects human-DPP4-transgenic mice
Luo-Yuan Xia[SUP] #[/SUP][SUP] 1 [/SUP], Zhen-Fei Wang[SUP] #[/SUP][SUP] 1 2 [/SUP], Xiao-Ming Cui[SUP] #[/SUP][SUP] 3 4 [/SUP], Yuan-Guo Li[SUP] #[/SUP][SUP] 2 [/SUP], Run-Ze Ye[SUP] #[/SUP][SUP] 1 [/SUP], Dai-Yun Zhu[SUP] 3 [/SUP], Fang-Xu Li[SUP] 2 [/SUP], Jie Zhang[SUP] 3 [/SUP], Wen-Hao Wang[SUP] 2 [/SUP], Ming-Zhu Zhang[SUP] 1 [/SUP], Wan-Ying Gao[SUP] 1 [/SUP], Lian-Feng Li[SUP] 1 [/SUP], Teng-Cheng Que[SUP] 5 [/SUP], Tie-Cheng Wang[SUP] 2 [/SUP], Na Jia[SUP] 3 4 [/SUP], Jia-Fu Jiang[SUP] 6 7 [/SUP], Yu-Wei Gao[SUP] 8 [/SUP], Wu-Chun Cao[SUP] 9 10 11 [/SUP]
Affiliations
- PMID: 38316817
- DOI: 10.1038/s41467-024-45453-2
We recently detected a HKU4-related coronavirus in subgenus Merbecovirus (named pangolin-CoV-HKU4-P251T) from a Malayan pangolin[SUP]1[/SUP]. Here we report isolation and characterization of pangolin-CoV-HKU4-P251T, the genome sequence of which is closest to that of a coronavirus from the greater bamboo bat (Tylonycteris robustula) in Yunnan Province, China, with a 94.3% nucleotide identity. Pangolin-CoV-HKU4-P251T is able to infect human cell lines, and replicates more efficiently in cells that express human-dipeptidyl-peptidase-4 (hDPP4)-expressing and pangolin-DPP4-expressing cells than in bat-DPP4-expressing cells. After intranasal inoculation with pangolin-CoV-HKU4-P251, hDPP4-transgenic female mice are likely infected, showing persistent viral RNA copy numbers in the lungs. Progressive interstitial pneumonia developed in the infected mice, characterized by the accumulation of macrophages, and increase of antiviral cytokines, proinflammatory cytokines, and chemokines in lung tissues. These findings suggest that the pangolin-borne HKU4-related coronavirus has a potential for emerging as a human pathogen by using hDPP4.