tetano
Editor, Senior Moderator
Nat Commun
. 2024 Oct 10;15(1):8770.
doi: 10.1038/s41467-024-53059-x. Multi-scenario surveillance of respiratory viruses in aerosols with sub-single-copy spatial resolution
Bao Li[SUP] #[/SUP][SUP] 1 2 [/SUP], Baobao Lin[SUP] #[/SUP][SUP] 1 [/SUP], Yan Wang[SUP] #[/SUP][SUP] 3 [/SUP], Ye Shi[SUP] #[/SUP][SUP] 4 [/SUP], Wu Zeng[SUP] 1 2 [/SUP], Yulan Zhao[SUP] 2 [/SUP], Yin Gu[SUP] 5 [/SUP], Chang Liu[SUP] 1 [/SUP], Hui Gao[SUP] 3 [/SUP], Hao Cheng[SUP] 3 [/SUP], Xiaoqun Zheng[SUP] 4 [/SUP], Guangxin Xiang[SUP] 6 [/SUP], Guiqiang Wang[SUP] 7 8 9 [/SUP], Peng Liu[SUP] 10 11 [/SUP]
Affiliations
Highly sensitive airborne virus monitoring is critical for preventing and containing epidemics. However, the detection of airborne viruses at ultra-low concentrations remains challenging due to the lack of ultra-sensitive methods and easy-to-deployment equipment. Here, we present an integrated microfluidic cartridge that can accurately detect SARS-COV-2, Influenza A, B, and respiratory syncytial virus with a sensitivity of 10 copies/mL. When integrated with a high-flow aerosol sampler, our microdevice can achieve a sub-single-copy spatial resolution of 0.83 copies/m[SUP]3[/SUP] for airborne virus surveillance with an air flow rate of 400 L/min and a sampling time of 30 minutes. We then designed a series of virus-in-aerosols monitoring systems (RIAMs), including versions of a multi-site sampling RIAMs (M-RIAMs), a stationary real-time RIAMs (S-RIAMs), and a roaming real-time RIAMs (R-RIAMs) for different application scenarios. Using M-RIAMs, we performed a comprehensive evaluation of 210 environmental samples from COVID-19 patient wards, including 30 aerosol samples. The highest positive detection rate of aerosol samples (60%) proved the aerosol-based SARS-CoV-2 monitoring represents an effective method for spatial risk assessment. The detection of 78 aerosol samples in real-world settings via S-RIAMs confirmed its reliability for ultra-sensitive and continuous airborne virus monitoring. Therefore, RIAMs shows the potential as an effective solution for mitigating the risk of airborne virus transmission.
. 2024 Oct 10;15(1):8770.
doi: 10.1038/s41467-024-53059-x. Multi-scenario surveillance of respiratory viruses in aerosols with sub-single-copy spatial resolution
Bao Li[SUP] #[/SUP][SUP] 1 2 [/SUP], Baobao Lin[SUP] #[/SUP][SUP] 1 [/SUP], Yan Wang[SUP] #[/SUP][SUP] 3 [/SUP], Ye Shi[SUP] #[/SUP][SUP] 4 [/SUP], Wu Zeng[SUP] 1 2 [/SUP], Yulan Zhao[SUP] 2 [/SUP], Yin Gu[SUP] 5 [/SUP], Chang Liu[SUP] 1 [/SUP], Hui Gao[SUP] 3 [/SUP], Hao Cheng[SUP] 3 [/SUP], Xiaoqun Zheng[SUP] 4 [/SUP], Guangxin Xiang[SUP] 6 [/SUP], Guiqiang Wang[SUP] 7 8 9 [/SUP], Peng Liu[SUP] 10 11 [/SUP]
Affiliations
- PMID: 39384836
- PMCID: PMC11464689
- DOI: 10.1038/s41467-024-53059-x
Highly sensitive airborne virus monitoring is critical for preventing and containing epidemics. However, the detection of airborne viruses at ultra-low concentrations remains challenging due to the lack of ultra-sensitive methods and easy-to-deployment equipment. Here, we present an integrated microfluidic cartridge that can accurately detect SARS-COV-2, Influenza A, B, and respiratory syncytial virus with a sensitivity of 10 copies/mL. When integrated with a high-flow aerosol sampler, our microdevice can achieve a sub-single-copy spatial resolution of 0.83 copies/m[SUP]3[/SUP] for airborne virus surveillance with an air flow rate of 400 L/min and a sampling time of 30 minutes. We then designed a series of virus-in-aerosols monitoring systems (RIAMs), including versions of a multi-site sampling RIAMs (M-RIAMs), a stationary real-time RIAMs (S-RIAMs), and a roaming real-time RIAMs (R-RIAMs) for different application scenarios. Using M-RIAMs, we performed a comprehensive evaluation of 210 environmental samples from COVID-19 patient wards, including 30 aerosol samples. The highest positive detection rate of aerosol samples (60%) proved the aerosol-based SARS-CoV-2 monitoring represents an effective method for spatial risk assessment. The detection of 78 aerosol samples in real-world settings via S-RIAMs confirmed its reliability for ultra-sensitive and continuous airborne virus monitoring. Therefore, RIAMs shows the potential as an effective solution for mitigating the risk of airborne virus transmission.