tetano
Editor, Senior Moderator
Nat Commun
. 2020 Aug 28;11(1):4355.
doi: 10.1038/s41467-020-18108-1.
Selective flexible packaging pathways of the segmented genome of influenza A virus
Ivan Haralampiev[SUP] 1 2 [/SUP], Simon Prisner[SUP] 1 [/SUP], Mor Nitzan[SUP] 3 [/SUP], Matthias Schade[SUP] 1 [/SUP], Fabian Jolmes[SUP] 1 4 [/SUP], Max Schreiber[SUP] 5 [/SUP], Maria Loidolt-Kr?ger[SUP] 6 4 [/SUP], Kalle Jongen[SUP] 1 [/SUP], Jasmine Chamiolo[SUP] 7 [/SUP], Niklaas Nilson[SUP] 1 [/SUP], Franziska Winter[SUP] 6 8 [/SUP], Nir Friedman[SUP] 3 9 [/SUP], Oliver Seitz[SUP] 7 [/SUP], Thorsten Wolff[SUP] 10 [/SUP], Andreas Herrmann[SUP] 11 [/SUP]
Affiliations
Abstract
The genome of influenza A viruses (IAV) is encoded in eight distinct viral ribonucleoproteins (vRNPs) that consist of negative sense viral RNA (vRNA) covered by the IAV nucleoprotein. Previous studies strongly support a selective packaging model by which vRNP segments are bundling to an octameric complex, which is integrated into budding virions. However, the pathway(s) generating a complete genome bundle is not known. We here use a multiplexed FISH assay to monitor all eight vRNAs in parallel in human lung epithelial cells. Analysis of 3.9 ? 10[SUP]5[/SUP] spots of colocalizing vRNAs provides quantitative insights into segment composition of vRNP complexes and, thus, implications for bundling routes. The complexes rarely contain multiple copies of a specific segment. The data suggest a selective packaging mechanism with limited flexibility by which vRNPs assemble into a complete IAV genome. We surmise that this flexibility forms an essential basis for the development of reassortant viruses with pandemic potential.
. 2020 Aug 28;11(1):4355.
doi: 10.1038/s41467-020-18108-1.
Selective flexible packaging pathways of the segmented genome of influenza A virus
Ivan Haralampiev[SUP] 1 2 [/SUP], Simon Prisner[SUP] 1 [/SUP], Mor Nitzan[SUP] 3 [/SUP], Matthias Schade[SUP] 1 [/SUP], Fabian Jolmes[SUP] 1 4 [/SUP], Max Schreiber[SUP] 5 [/SUP], Maria Loidolt-Kr?ger[SUP] 6 4 [/SUP], Kalle Jongen[SUP] 1 [/SUP], Jasmine Chamiolo[SUP] 7 [/SUP], Niklaas Nilson[SUP] 1 [/SUP], Franziska Winter[SUP] 6 8 [/SUP], Nir Friedman[SUP] 3 9 [/SUP], Oliver Seitz[SUP] 7 [/SUP], Thorsten Wolff[SUP] 10 [/SUP], Andreas Herrmann[SUP] 11 [/SUP]
Affiliations
- PMID: 32859915
- DOI: 10.1038/s41467-020-18108-1
Abstract
The genome of influenza A viruses (IAV) is encoded in eight distinct viral ribonucleoproteins (vRNPs) that consist of negative sense viral RNA (vRNA) covered by the IAV nucleoprotein. Previous studies strongly support a selective packaging model by which vRNP segments are bundling to an octameric complex, which is integrated into budding virions. However, the pathway(s) generating a complete genome bundle is not known. We here use a multiplexed FISH assay to monitor all eight vRNAs in parallel in human lung epithelial cells. Analysis of 3.9 ? 10[SUP]5[/SUP] spots of colocalizing vRNAs provides quantitative insights into segment composition of vRNP complexes and, thus, implications for bundling routes. The complexes rarely contain multiple copies of a specific segment. The data suggest a selective packaging mechanism with limited flexibility by which vRNPs assemble into a complete IAV genome. We surmise that this flexibility forms an essential basis for the development of reassortant viruses with pandemic potential.