tetano
Editor, Senior Moderator
Nat Commun
. 2020 Oct 16;11(1):5243.
doi: 10.1038/s41467-020-19080-6.
Whole blood immunophenotyping uncovers immature neutrophil-to-VD2 T-cell ratio as an early marker for severe COVID-19
Guillaume Carissimo[SUP] 1 2 [/SUP], Weili Xu[SUP] 3 [/SUP], Immanuel Kwok[SUP] 3 [/SUP], Mohammad Yazid Abdad[SUP] 4 [/SUP], Yi-Hao Chan[SUP] 5 3 [/SUP], Siew-Wai Fong[SUP] 5 3 6 [/SUP], Kia Joo Puan[SUP] 3 [/SUP], Cheryl Yi-Pin Lee[SUP] 5 3 [/SUP], Nicholas Kim-Wah Yeo[SUP] 5 3 [/SUP], Siti Naqiah Amrun[SUP] 5 3 [/SUP], Rhonda Sin-Ling Chee[SUP] 5 3 [/SUP], Wilson How[SUP] 3 [/SUP], Stephrene Chan[SUP] 7 8 9 10 [/SUP], Bingwen Eugene Fan[SUP] 7 8 9 10 [/SUP], Anand Kumar Andiappan[SUP] 3 [/SUP], Bernett Lee[SUP] 3 [/SUP], Olaf R?tzschke[SUP] 3 [/SUP], Barnaby Edward Young[SUP] 4 11 12 [/SUP], Yee-Sin Leo[SUP] 4 11 12 13 14 [/SUP], David Chien Lye[SUP] 4 11 12 13 [/SUP], Laurent Renia[SUP] 5 3 [/SUP], Lai Guan Ng[SUP] 3 [/SUP], Anis Larbi[SUP] 3 [/SUP], Lisa Fp Ng[SUP] 15 16 17 18 [/SUP]
Affiliations
Abstract
SARS-CoV-2 is the novel coronavirus responsible for the current COVID-19 pandemic. Severe complications are observed only in a small proportion of infected patients but the cellular mechanisms underlying this progression are still unknown. Comprehensive flow cytometry of whole blood samples from 54 COVID-19 patients reveals a dramatic increase in the number of immature neutrophils. This increase strongly correlates with disease severity and is associated with elevated IL-6 and IP-10 levels, two key players in the cytokine storm. The most pronounced decrease in cell counts is observed for CD8 T-cells and VD2 γδ T-cells, which both exhibit increased differentiation and activation. ROC analysis reveals that the count ratio of immature neutrophils to VD2 (or CD8) T-cells predicts pneumonia onset (0.9071) as well as hypoxia onset (0.8908) with high sensitivity and specificity. It would thus be a useful prognostic marker for preventive patient management and improved healthcare resource management.
. 2020 Oct 16;11(1):5243.
doi: 10.1038/s41467-020-19080-6.
Whole blood immunophenotyping uncovers immature neutrophil-to-VD2 T-cell ratio as an early marker for severe COVID-19
Guillaume Carissimo[SUP] 1 2 [/SUP], Weili Xu[SUP] 3 [/SUP], Immanuel Kwok[SUP] 3 [/SUP], Mohammad Yazid Abdad[SUP] 4 [/SUP], Yi-Hao Chan[SUP] 5 3 [/SUP], Siew-Wai Fong[SUP] 5 3 6 [/SUP], Kia Joo Puan[SUP] 3 [/SUP], Cheryl Yi-Pin Lee[SUP] 5 3 [/SUP], Nicholas Kim-Wah Yeo[SUP] 5 3 [/SUP], Siti Naqiah Amrun[SUP] 5 3 [/SUP], Rhonda Sin-Ling Chee[SUP] 5 3 [/SUP], Wilson How[SUP] 3 [/SUP], Stephrene Chan[SUP] 7 8 9 10 [/SUP], Bingwen Eugene Fan[SUP] 7 8 9 10 [/SUP], Anand Kumar Andiappan[SUP] 3 [/SUP], Bernett Lee[SUP] 3 [/SUP], Olaf R?tzschke[SUP] 3 [/SUP], Barnaby Edward Young[SUP] 4 11 12 [/SUP], Yee-Sin Leo[SUP] 4 11 12 13 14 [/SUP], David Chien Lye[SUP] 4 11 12 13 [/SUP], Laurent Renia[SUP] 5 3 [/SUP], Lai Guan Ng[SUP] 3 [/SUP], Anis Larbi[SUP] 3 [/SUP], Lisa Fp Ng[SUP] 15 16 17 18 [/SUP]
Affiliations
- PMID: 33067472
- DOI: 10.1038/s41467-020-19080-6
Abstract
SARS-CoV-2 is the novel coronavirus responsible for the current COVID-19 pandemic. Severe complications are observed only in a small proportion of infected patients but the cellular mechanisms underlying this progression are still unknown. Comprehensive flow cytometry of whole blood samples from 54 COVID-19 patients reveals a dramatic increase in the number of immature neutrophils. This increase strongly correlates with disease severity and is associated with elevated IL-6 and IP-10 levels, two key players in the cytokine storm. The most pronounced decrease in cell counts is observed for CD8 T-cells and VD2 γδ T-cells, which both exhibit increased differentiation and activation. ROC analysis reveals that the count ratio of immature neutrophils to VD2 (or CD8) T-cells predicts pneumonia onset (0.9071) as well as hypoxia onset (0.8908) with high sensitivity and specificity. It would thus be a useful prognostic marker for preventive patient management and improved healthcare resource management.