tetano
Editor, Senior Moderator
Nat Protoc
. 2020 Oct 26.
doi: 10.1038/s41596-020-00403-2. Online ahead of print.
Genetically modified mouse models to help fight COVID-19
Channabasavaiah B Gurumurthy[SUP] 1 2 [/SUP], Rolen M Quadros[SUP] 3 [/SUP], Guy P Richardson[SUP] 4 [/SUP], Larisa Y Poluektova[SUP] 5 [/SUP], Suzanne L Mansour[SUP] 6 [/SUP], Masato Ohtsuka[SUP] 7 8 [/SUP]
Affiliations
Abstract
The research community is in a race to understand the molecular mechanisms of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, to repurpose currently available antiviral drugs and to develop new therapies and vaccines against coronavirus disease 2019 (COVID-19). One major challenge in achieving these goals is the paucity of suitable preclinical animal models. Mice constitute ~70% of all the laboratory animal species used in biomedical research. Unfortunately, SARS-CoV-2 infects mice only if they have been genetically modified to express human ACE2. The inherent resistance of wild-type mice to SARS-CoV-2, combined with a wealth of genetic tools that are available only for modifying mice, offers a unique opportunity to create a versatile set of genetically engineered mouse models useful for COVID-19 research. We propose three broad categories of these models and more than two dozen designs that may be useful for SARS-CoV-2 research and for fighting COVID-19.
. 2020 Oct 26.
doi: 10.1038/s41596-020-00403-2. Online ahead of print.
Genetically modified mouse models to help fight COVID-19
Channabasavaiah B Gurumurthy[SUP] 1 2 [/SUP], Rolen M Quadros[SUP] 3 [/SUP], Guy P Richardson[SUP] 4 [/SUP], Larisa Y Poluektova[SUP] 5 [/SUP], Suzanne L Mansour[SUP] 6 [/SUP], Masato Ohtsuka[SUP] 7 8 [/SUP]
Affiliations
- PMID: 33106680
- DOI: 10.1038/s41596-020-00403-2
Abstract
The research community is in a race to understand the molecular mechanisms of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, to repurpose currently available antiviral drugs and to develop new therapies and vaccines against coronavirus disease 2019 (COVID-19). One major challenge in achieving these goals is the paucity of suitable preclinical animal models. Mice constitute ~70% of all the laboratory animal species used in biomedical research. Unfortunately, SARS-CoV-2 infects mice only if they have been genetically modified to express human ACE2. The inherent resistance of wild-type mice to SARS-CoV-2, combined with a wealth of genetic tools that are available only for modifying mice, offers a unique opportunity to create a versatile set of genetically engineered mouse models useful for COVID-19 research. We propose three broad categories of these models and more than two dozen designs that may be useful for SARS-CoV-2 research and for fighting COVID-19.