tetano
Editor, Senior Moderator
J Virol. 2014 Feb 12. [Epub ahead of print]
Naturally occurring mutations in the PA gene are key contributors to increased virulence of pandemic H1N1/09 influenza virus in mice.
Sun Y, Xu Q, Shen Y, Liu L, Wei K, Sun H, Pu J, Chang KC, Liu J.
Author information
Abstract
We examined the molecular basis of virulence of pandemic H1N1/09 influenza viruses by reverse genetics based on two H1N1/09 virus isolates (A/California/04/2009 [CA04] and A/swine/Shandong/731/2009 [SD731]) with contrasting pathogenicity in mice. We found that four amino acid mutations (PA-P224S, PB2-T588I, NA-V106I, and NS1-I123V) contributed to the lethal phenotype of SD731. Particularly, PA-P224S mutation when combined with PA-A70V in CA04 drastically reduced its 50% mouse lethal dose (LD50) by almost 1,000-fold.
PMID:
24522908
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24522908
Naturally occurring mutations in the PA gene are key contributors to increased virulence of pandemic H1N1/09 influenza virus in mice.
Sun Y, Xu Q, Shen Y, Liu L, Wei K, Sun H, Pu J, Chang KC, Liu J.
Author information
Abstract
We examined the molecular basis of virulence of pandemic H1N1/09 influenza viruses by reverse genetics based on two H1N1/09 virus isolates (A/California/04/2009 [CA04] and A/swine/Shandong/731/2009 [SD731]) with contrasting pathogenicity in mice. We found that four amino acid mutations (PA-P224S, PB2-T588I, NA-V106I, and NS1-I123V) contributed to the lethal phenotype of SD731. Particularly, PA-P224S mutation when combined with PA-A70V in CA04 drastically reduced its 50% mouse lethal dose (LD50) by almost 1,000-fold.
PMID:
24522908
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24522908