tetano
Editor, Senior Moderator
[h=1]A humanized MDCK cell line for the efficient isolation and propagation of human influenza viruses[/h]
[h=2]Abstract[/h] Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpressing (AX4) cells. A/H3N2 viruses propagated in hCK cells also maintained higher genetic stability than those propagated in MDCK and AX4 cells.
https://www.nature.com/articles/s41564-019-0433-6
- Kosuke Takada,
- Chiharu Kawakami,
- Shufang Fan,
- Shiho Chiba,
- Gongxun Zhong,
- Chunyang Gu,
- Kohei Shimizu,
- Sara Takasaki,
- Yuko Sakai-Tagawa,
- Tiago J. S. Lopes,
- Jayeeta Dutta,
- Zenab Khan,
- Divya Kriti,
- Harm van Bakel,
- Shinya Yamada,
- Tokiko Watanabe,
- Masaki Imai &
- Yoshihiro Kawaoka
[h=2]Abstract[/h] Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpressing (AX4) cells. A/H3N2 viruses propagated in hCK cells also maintained higher genetic stability than those propagated in MDCK and AX4 cells.
https://www.nature.com/articles/s41564-019-0433-6