tetano
Editor, Senior Moderator
Neuropathol Appl Neurobiol
. 2020 Sep 8.
doi: 10.1111/nan.12662. Online ahead of print.
Neuropathological Findings in Two Patients with Fatal COVID-19
Melanie P Jensen[SUP] 1 [/SUP], John Le Quesne[SUP] 2 3 [/SUP], Leah Officer-Jones[SUP] 2 [/SUP], Ana Teod?sio[SUP] 2 [/SUP], James Thaventhiran[SUP] 2 [/SUP], Catherine Ficken[SUP] 2 [/SUP], Martin Goddard[SUP] 4 [/SUP], Chris Smith[SUP] 5 [/SUP], David Menon[SUP] 6 [/SUP], Kieren S J Allinson[SUP] 7 [/SUP]
Affiliations
Abstract
Aims: To describe the neuropathological findings in two cases of fatal Coronavirus Disease 2019 (COVID-19) with neurological decline.
Methods: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection was confirmed in both patients by reverse transcription polymerase chain reaction (RT-PCR) from nasopharyngeal swabs antemortem. Coronial autopsies were performed on both patients and histological sampling of the brain was undertaken with a variety of histochemical and immunohistochemical stains. RNAscope? in situ hybridisation (ISH) using the V-nCoV2019-S probe and RT-PCR SARS-CoV-2 ribonucleic acid (RNA) was performed in paraffin-embedded brain tissue sampled from areas of pathology.
Results: Case 1 demonstrated severe multifocal cortical infarction with extensive perivascular calcification and numerous megakaryocytes, consistent with a severe multi-territorial cerebral vascular injury. There was associated cerebral thrombotic microangiopathy. Case 2 demonstrated a brainstem encephalitis centred on the dorsal medulla and a subacute regional infarct involving the cerebellar cortex. In both cases ISH and RT-PCR for SARS-CoV-2 RNA were negative in tissue sampled from the area of pathology.
Conclusions: Our case series adds calcifying cerebral cortical infarction with associated megakaryocytes and brainstem encephalitis to the spectrum of neuropathological findings that may contribute to the neurological decompensation seen in some COVID-19 patients. Viral RNA was not detected in post-mortem brain tissue, suggesting that these pathologies may not be a direct consequence of viral neuroinvasion and may represent para-infectious phenomena, relating to the systemic hyperinflammatory and hypercoagulable syndromes that both patients suffered.
Keywords: COVID-19; SARS-CoV-2; case series; neuropathology.
. 2020 Sep 8.
doi: 10.1111/nan.12662. Online ahead of print.
Neuropathological Findings in Two Patients with Fatal COVID-19
Melanie P Jensen[SUP] 1 [/SUP], John Le Quesne[SUP] 2 3 [/SUP], Leah Officer-Jones[SUP] 2 [/SUP], Ana Teod?sio[SUP] 2 [/SUP], James Thaventhiran[SUP] 2 [/SUP], Catherine Ficken[SUP] 2 [/SUP], Martin Goddard[SUP] 4 [/SUP], Chris Smith[SUP] 5 [/SUP], David Menon[SUP] 6 [/SUP], Kieren S J Allinson[SUP] 7 [/SUP]
Affiliations
- PMID: 32895961
- DOI: 10.1111/nan.12662
Abstract
Aims: To describe the neuropathological findings in two cases of fatal Coronavirus Disease 2019 (COVID-19) with neurological decline.
Methods: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection was confirmed in both patients by reverse transcription polymerase chain reaction (RT-PCR) from nasopharyngeal swabs antemortem. Coronial autopsies were performed on both patients and histological sampling of the brain was undertaken with a variety of histochemical and immunohistochemical stains. RNAscope? in situ hybridisation (ISH) using the V-nCoV2019-S probe and RT-PCR SARS-CoV-2 ribonucleic acid (RNA) was performed in paraffin-embedded brain tissue sampled from areas of pathology.
Results: Case 1 demonstrated severe multifocal cortical infarction with extensive perivascular calcification and numerous megakaryocytes, consistent with a severe multi-territorial cerebral vascular injury. There was associated cerebral thrombotic microangiopathy. Case 2 demonstrated a brainstem encephalitis centred on the dorsal medulla and a subacute regional infarct involving the cerebellar cortex. In both cases ISH and RT-PCR for SARS-CoV-2 RNA were negative in tissue sampled from the area of pathology.
Conclusions: Our case series adds calcifying cerebral cortical infarction with associated megakaryocytes and brainstem encephalitis to the spectrum of neuropathological findings that may contribute to the neurological decompensation seen in some COVID-19 patients. Viral RNA was not detected in post-mortem brain tissue, suggesting that these pathologies may not be a direct consequence of viral neuroinvasion and may represent para-infectious phenomena, relating to the systemic hyperinflammatory and hypercoagulable syndromes that both patients suffered.
Keywords: COVID-19; SARS-CoV-2; case series; neuropathology.