tetano
Editor, Senior Moderator
J Virol Methods. 2017 Mar 20. pii: S0166-0934(16)30210-5. doi: 10.1016/j.jviromet.2017.02.018. [Epub ahead of print]
[h=1]New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses.[/h] Naguib MM[SUP]1[/SUP], El-Kady MF[SUP]2[/SUP], L?schow D[SUP]3[/SUP], Hassan KE[SUP]2[/SUP], Arafa AS[SUP]4[/SUP], El-Zanaty A[SUP]4[/SUP], Hassan MK[SUP]4[/SUP], Hafez HM[SUP]3[/SUP], Grund C[SUP]5[/SUP], Harder TC[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.
Copyright ? 2017 Elsevier B.V. All rights reserved.
PMID: 28336367 DOI: 10.1016/j.jviromet.2017.02.018
[h=1]New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses.[/h] Naguib MM[SUP]1[/SUP], El-Kady MF[SUP]2[/SUP], L?schow D[SUP]3[/SUP], Hassan KE[SUP]2[/SUP], Arafa AS[SUP]4[/SUP], El-Zanaty A[SUP]4[/SUP], Hassan MK[SUP]4[/SUP], Hafez HM[SUP]3[/SUP], Grund C[SUP]5[/SUP], Harder TC[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.
Copyright ? 2017 Elsevier B.V. All rights reserved.
PMID: 28336367 DOI: 10.1016/j.jviromet.2017.02.018