tetano
Editor, Senior Moderator
Viruses. 2018 Sep 25;10(10). pii: E522. doi: 10.3390/v10100522.
[h=1]Non-Uniform and Non-Random Binding of Nucleoprotein to Influenza A and B Viral RNA.[/h] Le Sage V[SUP]1[/SUP], Nanni AV[SUP]2[/SUP], Bhagwat AR[SUP]3[/SUP], Snyder DJ[SUP]4,[/SUP][SUP]5[/SUP], Cooper VS[SUP]6,[/SUP][SUP]7[/SUP], Lakdawala SS[SUP]8[/SUP], Lee N[SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The genomes of influenza A and B viruses have eight, single-stranded RNA segments that exist in the form of a viral ribonucleoprotein complex in association with nucleoprotein (NP) and an RNA-dependent RNA polymerase complex. We previously used high-throughput RNA sequencing coupled with crosslinking immunoprecipitation (HITS-CLIP) to examine where NP binds to the viral RNA (vRNA) and demonstrated for two H1N1 strains that NP binds vRNA in a non-uniform, non-random manner. In this study, we expand on those initial observations and describe the NP-vRNA binding profile for a seasonal H3N2 and influenza B virus. We show that, similar to H1N1 strains, NP binds vRNA in a non-uniform and non-random manner. Each viral gene segment has a unique NP binding profile with areas that are enriched for NP association as well as free of NP-binding. Interestingly, NP-vRNA binding profiles have some conservation between influenza A viruses, H1N1 and H3N2, but no correlation was observed between influenza A and B viruses. Our study demonstrates the conserved nature of non-uniform NP binding within influenza viruses. Mapping of the NP-bound vRNA segments provides information on the flexible NP regions that may be involved in facilitating assembly.
[h=4]KEYWORDS:[/h] HITS-CLIP; influenza virus; nucleoprotein; viral RNA; viral ribonucleoprotein
PMID: 30257455 DOI: 10.3390/v10100522
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[h=1]Non-Uniform and Non-Random Binding of Nucleoprotein to Influenza A and B Viral RNA.[/h] Le Sage V[SUP]1[/SUP], Nanni AV[SUP]2[/SUP], Bhagwat AR[SUP]3[/SUP], Snyder DJ[SUP]4,[/SUP][SUP]5[/SUP], Cooper VS[SUP]6,[/SUP][SUP]7[/SUP], Lakdawala SS[SUP]8[/SUP], Lee N[SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The genomes of influenza A and B viruses have eight, single-stranded RNA segments that exist in the form of a viral ribonucleoprotein complex in association with nucleoprotein (NP) and an RNA-dependent RNA polymerase complex. We previously used high-throughput RNA sequencing coupled with crosslinking immunoprecipitation (HITS-CLIP) to examine where NP binds to the viral RNA (vRNA) and demonstrated for two H1N1 strains that NP binds vRNA in a non-uniform, non-random manner. In this study, we expand on those initial observations and describe the NP-vRNA binding profile for a seasonal H3N2 and influenza B virus. We show that, similar to H1N1 strains, NP binds vRNA in a non-uniform and non-random manner. Each viral gene segment has a unique NP binding profile with areas that are enriched for NP association as well as free of NP-binding. Interestingly, NP-vRNA binding profiles have some conservation between influenza A viruses, H1N1 and H3N2, but no correlation was observed between influenza A and B viruses. Our study demonstrates the conserved nature of non-uniform NP binding within influenza viruses. Mapping of the NP-bound vRNA segments provides information on the flexible NP regions that may be involved in facilitating assembly.
[h=4]KEYWORDS:[/h] HITS-CLIP; influenza virus; nucleoprotein; viral RNA; viral ribonucleoprotein
PMID: 30257455 DOI: 10.3390/v10100522
Free full text