tetano
Editor, Senior Moderator
J Clin Virol. 2015 Apr;65:58-61. doi: 10.1016/j.jcv.2015.02.007. Epub 2015 Feb 11.
[h=1]Novel reassortant H10N7 avian influenza viruses isolated from chickens in Eastern China.[/h] Wu H[SUP]1[/SUP], Lu R[SUP]2[/SUP], Wu X[SUP]3[/SUP], Peng X[SUP]3[/SUP], Xu L[SUP]4[/SUP], Cheng L[SUP]3[/SUP], Lu X[SUP]3[/SUP], Jin C[SUP]3[/SUP], Xie T[SUP]3[/SUP], Yao H[SUP]3[/SUP], Wu N[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Since 2004, the H10N7 subtype avian influenza virus (AIV) has caused sporadic human infections with variable clinical symptoms world-wide. However, there is limited information pertaining to the molecular characteristics of H10N7 AIVs in China.
[h=4]OBJECTIVE:[/h] To more fully characterize the genetic relationships between three novel H10N7 strains isolated from chickens in Eastern China and the strains isolated from birds throughout Asia, and to determine the pathogenicity of the H10N7 isolates in vivo.
[h=4]STUDY DESIGN:[/h] All eight gene segments from the Chinese H10N7 strains were sequenced and compared with AIV strains available in GenBank. The virulence of the three isolates was determined in chickens and mice.
[h=4]RESULTS:[/h] Three H10N7 subtype avian influenza viruses were isolated from chickens in live poultry markets in Eastern China in 2014: (1) A/chicken/Zhejiang/2C66/2014(H10N7) (ZJ-2C66), (2) A/chicken/Zhejiang/2CP2/2014(H10N7) (ZJ-2CP2), and (3) A/chicken/Zhejiang/2CP8/2014(H10N7) (ZJ-2CP8). Phylogenetic analysis indicated that the viruses contained genetic material from H10, H2, H7, and H3 AIV strains that were circulating at the same time. The reassortant H10N7 viruses were found to be minimally pathogenic in chickens and moderately pathogenic in mice. The viruses were able to replicate in mice without prior adaptation.
[h=4]CONCLUSION:[/h] These results suggest that H10N7 surveillance in poultry should be used as an early warning system for avian influenza outbreaks. The novel strains identified here may post a threat to human health in the future if they continue to circulate.
Copyright ? 2015 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Avian influenza virus; Chickens; Eastern China; H10N7; Poultry; Virus reassortment
PMID: 25766990 [PubMed - in process]
[h=1]Novel reassortant H10N7 avian influenza viruses isolated from chickens in Eastern China.[/h] Wu H[SUP]1[/SUP], Lu R[SUP]2[/SUP], Wu X[SUP]3[/SUP], Peng X[SUP]3[/SUP], Xu L[SUP]4[/SUP], Cheng L[SUP]3[/SUP], Lu X[SUP]3[/SUP], Jin C[SUP]3[/SUP], Xie T[SUP]3[/SUP], Yao H[SUP]3[/SUP], Wu N[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Since 2004, the H10N7 subtype avian influenza virus (AIV) has caused sporadic human infections with variable clinical symptoms world-wide. However, there is limited information pertaining to the molecular characteristics of H10N7 AIVs in China.
[h=4]OBJECTIVE:[/h] To more fully characterize the genetic relationships between three novel H10N7 strains isolated from chickens in Eastern China and the strains isolated from birds throughout Asia, and to determine the pathogenicity of the H10N7 isolates in vivo.
[h=4]STUDY DESIGN:[/h] All eight gene segments from the Chinese H10N7 strains were sequenced and compared with AIV strains available in GenBank. The virulence of the three isolates was determined in chickens and mice.
[h=4]RESULTS:[/h] Three H10N7 subtype avian influenza viruses were isolated from chickens in live poultry markets in Eastern China in 2014: (1) A/chicken/Zhejiang/2C66/2014(H10N7) (ZJ-2C66), (2) A/chicken/Zhejiang/2CP2/2014(H10N7) (ZJ-2CP2), and (3) A/chicken/Zhejiang/2CP8/2014(H10N7) (ZJ-2CP8). Phylogenetic analysis indicated that the viruses contained genetic material from H10, H2, H7, and H3 AIV strains that were circulating at the same time. The reassortant H10N7 viruses were found to be minimally pathogenic in chickens and moderately pathogenic in mice. The viruses were able to replicate in mice without prior adaptation.
[h=4]CONCLUSION:[/h] These results suggest that H10N7 surveillance in poultry should be used as an early warning system for avian influenza outbreaks. The novel strains identified here may post a threat to human health in the future if they continue to circulate.
Copyright ? 2015 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Avian influenza virus; Chickens; Eastern China; H10N7; Poultry; Virus reassortment
PMID: 25766990 [PubMed - in process]