tetano
Editor, Senior Moderator
J Gen Virol. 2016 Nov 11. doi: 10.1099/jgv.0.000657. [Epub ahead of print]
[h=1]Novel reassortant H9N2 viruses in pigeons and evidence for antigenic diversity of H9N2 viruses isolated from quails in Egypt.[/h] Kandeil A[SUP]1[/SUP], El-Shesheny R[SUP]2[/SUP], Maatouq A[SUP]3[/SUP], Moatasim Y[SUP]4[/SUP], Cai Z[SUP]5[/SUP], McKenzie P[SUP]6[/SUP], Webby R[SUP]7[/SUP], Kayali G[SUP]8[/SUP], Ali MA[SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The endemicity of avian influenza viruses (AIVs) among Egyptian poultry represents a public health risk. Co-circulation of low pathogenic AIV H9N2 subtype with highly pathogenic AIV H5N1 subtype in Egyptian farms potentiates the possibility to generate novel reassortant viruses. Herein, the genetic characteristics of surface glycoproteins of 59 Egyptian H9N2 viruses, isolated between 2013 and 2015, were analyzed. To elucidate the potential of genetic reassortment, 10 H9N2 isolates were selected based on different avian hosts (chickens, ducks, pigeons and quails) and conducted for phylogenetic analyses of full genome sequences. Additionally, we performed antigenic analysis to further investigate the antigenic evolution of H9N2 viruses isolated during 2011-2015 in Egypt. Different viral characteristics including receptor binding affinity and drug resistance of representative Egyptian H9N2 viruses were further investigated. The surface glycoproteins of current Egyptian H9N2 viruses were completely closely related to viruses of the G1-like lineage isolated from Egypt. Analysis of full genome of 10 H9N2 isolates indicated that 2 pigeon isolates inherited 5 internal genes from Eurasian AIVs circulating in wild birds. Multiple peculiar mutations in virus internal genes were detected which probably permits transmission and virulence in mammals. Antigenic conservation of different Egyptian H9N2 isolates from chickens, pigeons, and ducks was observed, whereas quail isolates showed antigenic drift. The Egyptian H9N2 viruses preferentially bound to the human-like receptor rather than avian-like receptor. Our results suggest that the endemic H9N2 viruses in Egypt contain elements that may favor avian to human transmission and thus represent a public health risk.
PMID: 27902350 DOI: 10.1099/jgv.0.000657
[PubMed - as supplied by publisher]
[h=1]Novel reassortant H9N2 viruses in pigeons and evidence for antigenic diversity of H9N2 viruses isolated from quails in Egypt.[/h] Kandeil A[SUP]1[/SUP], El-Shesheny R[SUP]2[/SUP], Maatouq A[SUP]3[/SUP], Moatasim Y[SUP]4[/SUP], Cai Z[SUP]5[/SUP], McKenzie P[SUP]6[/SUP], Webby R[SUP]7[/SUP], Kayali G[SUP]8[/SUP], Ali MA[SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The endemicity of avian influenza viruses (AIVs) among Egyptian poultry represents a public health risk. Co-circulation of low pathogenic AIV H9N2 subtype with highly pathogenic AIV H5N1 subtype in Egyptian farms potentiates the possibility to generate novel reassortant viruses. Herein, the genetic characteristics of surface glycoproteins of 59 Egyptian H9N2 viruses, isolated between 2013 and 2015, were analyzed. To elucidate the potential of genetic reassortment, 10 H9N2 isolates were selected based on different avian hosts (chickens, ducks, pigeons and quails) and conducted for phylogenetic analyses of full genome sequences. Additionally, we performed antigenic analysis to further investigate the antigenic evolution of H9N2 viruses isolated during 2011-2015 in Egypt. Different viral characteristics including receptor binding affinity and drug resistance of representative Egyptian H9N2 viruses were further investigated. The surface glycoproteins of current Egyptian H9N2 viruses were completely closely related to viruses of the G1-like lineage isolated from Egypt. Analysis of full genome of 10 H9N2 isolates indicated that 2 pigeon isolates inherited 5 internal genes from Eurasian AIVs circulating in wild birds. Multiple peculiar mutations in virus internal genes were detected which probably permits transmission and virulence in mammals. Antigenic conservation of different Egyptian H9N2 isolates from chickens, pigeons, and ducks was observed, whereas quail isolates showed antigenic drift. The Egyptian H9N2 viruses preferentially bound to the human-like receptor rather than avian-like receptor. Our results suggest that the endemic H9N2 viruses in Egypt contain elements that may favor avian to human transmission and thus represent a public health risk.
PMID: 27902350 DOI: 10.1099/jgv.0.000657
[PubMed - as supplied by publisher]