tetano
Editor, Senior Moderator
Mol Ther Nucleic Acids. 2019 May 15;17:10-23. doi: 10.1016/j.omtn.2019.04.028. [Epub ahead of print]
[h=1]Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus.[/h] Huang SY[SUP]1[/SUP], Huang CH[SUP]2[/SUP], Chen CJ[SUP]3[/SUP], Chen TW[SUP]4[/SUP], Lin CY[SUP]5[/SUP], Lin YT[SUP]6[/SUP], Kuo SM[SUP]3[/SUP], Huang CG[SUP]7[/SUP], Lee LA[SUP]8[/SUP], Chen YH[SUP]1[/SUP], Chen MF[SUP]3[/SUP], Kuo RL[SUP]9[/SUP], Shih SR[SUP]10[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The role of microRNA (miRNA) in influenza A virus (IAV) host species specificity is not well understood as yet. Here, we show that a host miRNA, miR-1290, is induced through the extracellular signal-regulated kinase (ERK) pathway upon IAV infection and is associated with increased viral titers in human cells and ferret animal models. miR-1290 was observed to target and reduce expression of the host vimentin gene. Vimentin binds with the PB2 subunit of influenza A virus ribonucleoprotein (vRNP), and knockdown of vimentin expression significantly increased vRNP nuclear retention and viral polymerase activity. Interestingly, miR-1290 was not detected in either chicken cells or mouse animal models, and the 3' UTR of the chicken vimentin gene contains no binding site for miR-1290. These findings point to a host species-specific mechanism by which IAV upregulates miR-1290 to disrupt vimentin expression and retain vRNP in the nucleus, thereby enhancing viral polymerase activity and viral replication.
Copyright ? 2019. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] ferret; host species-specificity; influenza A virus; miR-1290; miRNA; vRNP; vimentin; viral ribonucleoprotein; virus
PMID: 31173947 PMCID: PMC6554369 DOI: 10.1016/j.omtn.2019.04.028
[h=1]Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus.[/h] Huang SY[SUP]1[/SUP], Huang CH[SUP]2[/SUP], Chen CJ[SUP]3[/SUP], Chen TW[SUP]4[/SUP], Lin CY[SUP]5[/SUP], Lin YT[SUP]6[/SUP], Kuo SM[SUP]3[/SUP], Huang CG[SUP]7[/SUP], Lee LA[SUP]8[/SUP], Chen YH[SUP]1[/SUP], Chen MF[SUP]3[/SUP], Kuo RL[SUP]9[/SUP], Shih SR[SUP]10[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The role of microRNA (miRNA) in influenza A virus (IAV) host species specificity is not well understood as yet. Here, we show that a host miRNA, miR-1290, is induced through the extracellular signal-regulated kinase (ERK) pathway upon IAV infection and is associated with increased viral titers in human cells and ferret animal models. miR-1290 was observed to target and reduce expression of the host vimentin gene. Vimentin binds with the PB2 subunit of influenza A virus ribonucleoprotein (vRNP), and knockdown of vimentin expression significantly increased vRNP nuclear retention and viral polymerase activity. Interestingly, miR-1290 was not detected in either chicken cells or mouse animal models, and the 3' UTR of the chicken vimentin gene contains no binding site for miR-1290. These findings point to a host species-specific mechanism by which IAV upregulates miR-1290 to disrupt vimentin expression and retain vRNP in the nucleus, thereby enhancing viral polymerase activity and viral replication.
Copyright ? 2019. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] ferret; host species-specificity; influenza A virus; miR-1290; miRNA; vRNP; vimentin; viral ribonucleoprotein; virus
PMID: 31173947 PMCID: PMC6554369 DOI: 10.1016/j.omtn.2019.04.028