tetano
Editor, Senior Moderator
NPJ Vaccines
. 2024 Nov 19;9(1):228.
doi: 10.1038/s41541-024-01011-x. Boosting neuraminidase immunity in the presence of hemagglutinin with the next generation of influenza vaccines
Guadalupe Cortés[SUP] 1 [/SUP], Irina Ustyugova[SUP] 2 [/SUP], Timothy Farrell[SUP] 2 [/SUP], Clint McDaniel[SUP] 2 [/SUP], Colleen Britain[SUP] 2 [/SUP], Christopher Romano[SUP] 3 [/SUP], Siré N'Diaye[SUP] 4 [/SUP], Lingyi Zheng[SUP] 4 [/SUP], Mithila Ferdous[SUP] 3 [/SUP], Justin Iampietro[SUP] 2 [/SUP], Svetlana Pougatcheva[SUP] 2 [/SUP], Lauren La Rue[SUP] 2 [/SUP], Liqun Han[SUP] 2 [/SUP], Fuqin Ma[SUP] 2 [/SUP], Svetlana Stegalkina[SUP] 2 [/SUP], Satyajit Ray[SUP] 2 [/SUP], Jianxin Zhang[SUP] 2 [/SUP], Mario Barro[SUP] 2 [/SUP]
Affiliations
Neuraminidase (NA), the second most abundant surface glycoprotein on the influenza virus, plays a key role in viral replication and propagation. Despite growing evidence showing that NA-specific antibodies correlate with resistance to disease in humans, current licensed vaccines focus almost entirely on the hemagglutinin (HA) antigen. Here, we demonstrate that recombinant NA (rNA) protein is highly immunogenic in both naïve mice and ferrets, as well as in pre-immune ferrets, irrespective of the level of match with preexisting immunity. Ferrets vaccinated with rNA developed mild influenza disease symptoms upon challenge with human H3N2 influenza virus, and anti-NA antibody responses appeared correlated with reduction in disease severity. The addition of rNA to a quadrivalent HA-based vaccine induced robust NA-specific humoral immunity in ferrets, while retaining the ability to induce HA-specific immunity. These results demonstrate that the addition of rNA is a viable option to increase immunogenicity and potentially efficacy versus currently licensed influenza vaccines by means of boosting NA immunity.
. 2024 Nov 19;9(1):228.
doi: 10.1038/s41541-024-01011-x. Boosting neuraminidase immunity in the presence of hemagglutinin with the next generation of influenza vaccines
Guadalupe Cortés[SUP] 1 [/SUP], Irina Ustyugova[SUP] 2 [/SUP], Timothy Farrell[SUP] 2 [/SUP], Clint McDaniel[SUP] 2 [/SUP], Colleen Britain[SUP] 2 [/SUP], Christopher Romano[SUP] 3 [/SUP], Siré N'Diaye[SUP] 4 [/SUP], Lingyi Zheng[SUP] 4 [/SUP], Mithila Ferdous[SUP] 3 [/SUP], Justin Iampietro[SUP] 2 [/SUP], Svetlana Pougatcheva[SUP] 2 [/SUP], Lauren La Rue[SUP] 2 [/SUP], Liqun Han[SUP] 2 [/SUP], Fuqin Ma[SUP] 2 [/SUP], Svetlana Stegalkina[SUP] 2 [/SUP], Satyajit Ray[SUP] 2 [/SUP], Jianxin Zhang[SUP] 2 [/SUP], Mario Barro[SUP] 2 [/SUP]
Affiliations
- PMID: 39562599
- PMCID: PMC11577023
- DOI: 10.1038/s41541-024-01011-x
Neuraminidase (NA), the second most abundant surface glycoprotein on the influenza virus, plays a key role in viral replication and propagation. Despite growing evidence showing that NA-specific antibodies correlate with resistance to disease in humans, current licensed vaccines focus almost entirely on the hemagglutinin (HA) antigen. Here, we demonstrate that recombinant NA (rNA) protein is highly immunogenic in both naïve mice and ferrets, as well as in pre-immune ferrets, irrespective of the level of match with preexisting immunity. Ferrets vaccinated with rNA developed mild influenza disease symptoms upon challenge with human H3N2 influenza virus, and anti-NA antibody responses appeared correlated with reduction in disease severity. The addition of rNA to a quadrivalent HA-based vaccine induced robust NA-specific humoral immunity in ferrets, while retaining the ability to induce HA-specific immunity. These results demonstrate that the addition of rNA is a viable option to increase immunogenicity and potentially efficacy versus currently licensed influenza vaccines by means of boosting NA immunity.