tetano
Editor, Senior Moderator
NPJ Vaccines
. 2022 Aug 30;7(1):103.
doi: 10.1038/s41541-022-00524-7.
The antibody landscapes following AS03 and MF59 adjuvanted H5N1 vaccination
Johannes B Goll[SUP] 1 [/SUP], Aarti Jain[SUP] 2 [/SUP], Travis L Jensen[SUP] 1 [/SUP], Rafael Assis[SUP] 2 [/SUP], Rie Nakajima[SUP] 2 [/SUP], Algis Jasinskas[SUP] 2 [/SUP], Lynda Coughlan[SUP] 3 4 [/SUP], Sami R Cherikh[SUP] 1 [/SUP], Casey E Gelber[SUP] 1 [/SUP], S Khan[SUP] 2 [/SUP], D Huw Davies[SUP] 2 [/SUP], Philip Meade[SUP] 5 [/SUP], Daniel Stadlbauer[SUP] 5 6 [/SUP], Shirin Strohmeier[SUP] 5 [/SUP], Florian Krammer[SUP] 5 [/SUP], Wilbur H Chen[SUP] 3 [/SUP], Philip L Felgner[SUP] 7 [/SUP]
Affiliations
Abstract
Current seasonal and pre-pandemic influenza vaccines induce short-lived predominantly strain-specific and limited heterosubtypic responses. To better understand how vaccine adjuvants AS03 and MF59 may provide improved antibody responses to vaccination, we interrogated serum from subjects who received 2 doses of inactivated monovalent influenza A/Indonesia/05/2005 vaccine with or without AS03 or MF59 using hemagglutinin (HA) microarrays (NCT01317758 and NCT01317745). The arrays were designed to reflect both full-length and globular head HA derived from 17 influenza A subtypes (H1 to H16 and H18) and influenza B strains. We observed significantly increased strain-specific and broad homo- and heterosubtypic antibody responses with both AS03 and MF59 adjuvanted vaccination with AS03 achieving a higher titer and breadth of IgG responses relative to MF59. The adjuvanted vaccine was also associated with the elicitation of stalk-directed antibody. We established good correlation of the array antibody responses to H5 antigens with standard HA inhibition and microneutralization titers.
. 2022 Aug 30;7(1):103.
doi: 10.1038/s41541-022-00524-7.
The antibody landscapes following AS03 and MF59 adjuvanted H5N1 vaccination
Johannes B Goll[SUP] 1 [/SUP], Aarti Jain[SUP] 2 [/SUP], Travis L Jensen[SUP] 1 [/SUP], Rafael Assis[SUP] 2 [/SUP], Rie Nakajima[SUP] 2 [/SUP], Algis Jasinskas[SUP] 2 [/SUP], Lynda Coughlan[SUP] 3 4 [/SUP], Sami R Cherikh[SUP] 1 [/SUP], Casey E Gelber[SUP] 1 [/SUP], S Khan[SUP] 2 [/SUP], D Huw Davies[SUP] 2 [/SUP], Philip Meade[SUP] 5 [/SUP], Daniel Stadlbauer[SUP] 5 6 [/SUP], Shirin Strohmeier[SUP] 5 [/SUP], Florian Krammer[SUP] 5 [/SUP], Wilbur H Chen[SUP] 3 [/SUP], Philip L Felgner[SUP] 7 [/SUP]
Affiliations
- PMID: 36042229
- DOI: 10.1038/s41541-022-00524-7
Abstract
Current seasonal and pre-pandemic influenza vaccines induce short-lived predominantly strain-specific and limited heterosubtypic responses. To better understand how vaccine adjuvants AS03 and MF59 may provide improved antibody responses to vaccination, we interrogated serum from subjects who received 2 doses of inactivated monovalent influenza A/Indonesia/05/2005 vaccine with or without AS03 or MF59 using hemagglutinin (HA) microarrays (NCT01317758 and NCT01317745). The arrays were designed to reflect both full-length and globular head HA derived from 17 influenza A subtypes (H1 to H16 and H18) and influenza B strains. We observed significantly increased strain-specific and broad homo- and heterosubtypic antibody responses with both AS03 and MF59 adjuvanted vaccination with AS03 achieving a higher titer and breadth of IgG responses relative to MF59. The adjuvanted vaccine was also associated with the elicitation of stalk-directed antibody. We established good correlation of the array antibody responses to H5 antigens with standard HA inhibition and microneutralization titers.