tetano
Editor, Senior Moderator
Clin Exp Vaccine Res. 2019 Jan;8(1):54-63. doi: 10.7774/cevr.2019.8.1.54. Epub 2019 Jan 31.
[h=1]Nucleoprotein vaccine induces cross-protective cytotoxic T lymphocytes against both lineages of influenza B virus.[/h] Lee SY[SUP]1[/SUP], Kang JO[SUP]1[/SUP], Chang J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]Purpose:[/h] The influenza B virus diverges into two antigenically distinct lineages: B/Yamagata and B/Victoria. Influenza B is the dominant circulating virus during some influenza seasons, and recent data demonstrated that influenza A and B infection similarly cause severe clinical symptoms in hospitalized patients. Nucleoprotein (NP) is a good target for a universal influenza vaccine. This study investigated whether NP epitope variation within two lineages affects the dominant cytotoxic T lymphocyte (CTL) responses induced by vaccination and the resultant protective immunity.
[h=4]Materials and Methods:[/h] The NP of B/Yamagata/16/1988, the representative strain of the Yamagata lineage, includes a dominant CTL epitope, FSPIRITFL, while B/Shangdong/7/1997 from the Victoria lineage has one amino acid difference in this sequence, FSPIRVTFL. Two recombinant replication-deficient adenovirus (rAd)-vectored vaccines expressing either NP were prepared (rAd/B-NP(I) and rAd/B-NP(V), respectively) and administered to BALB/c mice intranasally. To examine the efficacy of vaccination, antibody responses, CTL responses, and morbidity/mortality after challenge were measured.
[h=4]Results:[/h] Both vaccines induce similar antibody and CD8 T-cell responses cross-reacting to both epitopes, and also confer cross-protection against both lineages regardless of amino acid difference.
[h=4]Conclusion:[/h] The rAd-vectored vaccine expressing the NP could be developed as universal influenza B vaccine which provides broader protection.
[h=4]KEYWORDS:[/h] Cross protective immunity; Cytotoxic T lymphocytes; Epitope; Influenza B virus; Nucleoproteins; Recombinant adenovirus
PMID: 30775351 PMCID: PMC6369129 DOI: 10.7774/cevr.2019.8.1.54
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[h=1]Nucleoprotein vaccine induces cross-protective cytotoxic T lymphocytes against both lineages of influenza B virus.[/h] Lee SY[SUP]1[/SUP], Kang JO[SUP]1[/SUP], Chang J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]Purpose:[/h] The influenza B virus diverges into two antigenically distinct lineages: B/Yamagata and B/Victoria. Influenza B is the dominant circulating virus during some influenza seasons, and recent data demonstrated that influenza A and B infection similarly cause severe clinical symptoms in hospitalized patients. Nucleoprotein (NP) is a good target for a universal influenza vaccine. This study investigated whether NP epitope variation within two lineages affects the dominant cytotoxic T lymphocyte (CTL) responses induced by vaccination and the resultant protective immunity.
[h=4]Materials and Methods:[/h] The NP of B/Yamagata/16/1988, the representative strain of the Yamagata lineage, includes a dominant CTL epitope, FSPIRITFL, while B/Shangdong/7/1997 from the Victoria lineage has one amino acid difference in this sequence, FSPIRVTFL. Two recombinant replication-deficient adenovirus (rAd)-vectored vaccines expressing either NP were prepared (rAd/B-NP(I) and rAd/B-NP(V), respectively) and administered to BALB/c mice intranasally. To examine the efficacy of vaccination, antibody responses, CTL responses, and morbidity/mortality after challenge were measured.
[h=4]Results:[/h] Both vaccines induce similar antibody and CD8 T-cell responses cross-reacting to both epitopes, and also confer cross-protection against both lineages regardless of amino acid difference.
[h=4]Conclusion:[/h] The rAd-vectored vaccine expressing the NP could be developed as universal influenza B vaccine which provides broader protection.
[h=4]KEYWORDS:[/h] Cross protective immunity; Cytotoxic T lymphocytes; Epitope; Influenza B virus; Nucleoproteins; Recombinant adenovirus
PMID: 30775351 PMCID: PMC6369129 DOI: 10.7774/cevr.2019.8.1.54
Free full text