tetano
Editor, Senior Moderator
Nucleosides Nucleotides Nucleic Acids
. 2023 Jan 28;1-15.
doi: 10.1080/15257770.2023.2172183. Online ahead of print.
The effect of DNA repair gene variants on COVID-19 disease: susceptibility, severity, and clinical course
Naci Senkal[SUP] 1 [/SUP], Istemi Serin[SUP] 2 [/SUP], Sacide Pehlivan[SUP] 3 [/SUP], Mustafa Pehlivan[SUP] 2 [/SUP], Alpay Medetalibeyoglu[SUP] 1 [/SUP], Timurhan Cebeci[SUP] 1 [/SUP], Hilal Konyaoglu[SUP] 1 [/SUP], Yasemin Oyacı[SUP] 3 [/SUP], Gozde Yesil Sayın[SUP] 4 [/SUP], Ummuhan Isoglu-Alkac[SUP] 5 [/SUP], Tufan Tukek[SUP] 1 [/SUP], Murat Kose[SUP] 1 [/SUP]
Affiliations
Abstract
Oxidative stress (OS), which leads to DNA damage, plays a role in the pathogenesis of Coronavirus disease 2019 (COVID-19). We aimed to evaluate the role of DNA repair gene variants [X-ray repair cross complementing 4 (XRCC4) rs28360071, rs6869366, and X-ray cross-complementary gene 1 (XRCC1) rs25487] in susceptibility to COVID-19 in a Turkish population. We also evaluated its effect on the clinical course of the disease. A total of 300 subjects, including 200 COVID-19 patients and 100 healthy controls, were included in this study. These variants were genotyped using polymerase chain reaction (PCR) and/or PCR-restriction fragment length polymorphism (RFLP) methods. The patients were divided into three groups: those with a mild or severe infection; those who died or lived at the 28-day follow-up; those who required inpatient treatment or intensive care. There were 87 women (43.5%) and 113 men (56.5%) in the patient group. Hypertension was the most common comorbidity (26%). In the patient group, XRCC4 rs6869366 G/G genotype and G allele frequency were increased compared to controls, while XRCC4 rs6869366 G/T and T/T genotype frequencies were found to be higher in controls compared to patients. For XRCC1 rs25487, the A/A and A/G genotypes were significantly associated with COVID-19 disease. All of the patients hospitalized in the intensive care unit had the XRCC4 rs6869366 G/G genotype. In this study, we evaluated for the first time the impact of DNA repair gene variants on COVID-19 susceptibility. Results suggested that XRCC4 rs6869366 and XRCC1 rs25487 were associated with COVID-19 suspectibility and clinical course.
Keywords: COVID-19; DNA repair; PCR; RFLP; XRCC1; XRCC4.
. 2023 Jan 28;1-15.
doi: 10.1080/15257770.2023.2172183. Online ahead of print.
The effect of DNA repair gene variants on COVID-19 disease: susceptibility, severity, and clinical course
Naci Senkal[SUP] 1 [/SUP], Istemi Serin[SUP] 2 [/SUP], Sacide Pehlivan[SUP] 3 [/SUP], Mustafa Pehlivan[SUP] 2 [/SUP], Alpay Medetalibeyoglu[SUP] 1 [/SUP], Timurhan Cebeci[SUP] 1 [/SUP], Hilal Konyaoglu[SUP] 1 [/SUP], Yasemin Oyacı[SUP] 3 [/SUP], Gozde Yesil Sayın[SUP] 4 [/SUP], Ummuhan Isoglu-Alkac[SUP] 5 [/SUP], Tufan Tukek[SUP] 1 [/SUP], Murat Kose[SUP] 1 [/SUP]
Affiliations
- PMID: 36708261
- DOI: 10.1080/15257770.2023.2172183
Abstract
Oxidative stress (OS), which leads to DNA damage, plays a role in the pathogenesis of Coronavirus disease 2019 (COVID-19). We aimed to evaluate the role of DNA repair gene variants [X-ray repair cross complementing 4 (XRCC4) rs28360071, rs6869366, and X-ray cross-complementary gene 1 (XRCC1) rs25487] in susceptibility to COVID-19 in a Turkish population. We also evaluated its effect on the clinical course of the disease. A total of 300 subjects, including 200 COVID-19 patients and 100 healthy controls, were included in this study. These variants were genotyped using polymerase chain reaction (PCR) and/or PCR-restriction fragment length polymorphism (RFLP) methods. The patients were divided into three groups: those with a mild or severe infection; those who died or lived at the 28-day follow-up; those who required inpatient treatment or intensive care. There were 87 women (43.5%) and 113 men (56.5%) in the patient group. Hypertension was the most common comorbidity (26%). In the patient group, XRCC4 rs6869366 G/G genotype and G allele frequency were increased compared to controls, while XRCC4 rs6869366 G/T and T/T genotype frequencies were found to be higher in controls compared to patients. For XRCC1 rs25487, the A/A and A/G genotypes were significantly associated with COVID-19 disease. All of the patients hospitalized in the intensive care unit had the XRCC4 rs6869366 G/G genotype. In this study, we evaluated for the first time the impact of DNA repair gene variants on COVID-19 susceptibility. Results suggested that XRCC4 rs6869366 and XRCC1 rs25487 were associated with COVID-19 suspectibility and clinical course.
Keywords: COVID-19; DNA repair; PCR; RFLP; XRCC1; XRCC4.