tetano
Editor, Senior Moderator
PLoS One. 2013 Jul 11;8(7):e69121. doi: 10.1371/journal.pone.0069121. Print 2013.
Observations of immuno-gold conjugates on influenza viruses using waveguide-mode sensors.
Gopinath SC, Awazu K, Fujimaki M, Shimizu K, Shima T.
Source
Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Abstract
Gold nanoparticles were conjugated to an antibody (immuno-AuNP) against A/Udorn/307/1972 (H3N2) influenza virus to detect viruses on a sensing plate designed for an evanescent field-coupled waveguide-mode sensor. Experiments were conducted using human influenza A/H3N2 strains, and immuno-AuNP could detect 8?10(5) PFU/ml (40 pg/?l) intact A/Udorn/307/1972 and 120 pg/?l A/Brisbane/10/2007. Furthermore, increased signal magnitude was achieved in the presence of non-ionic detergent, as the virtual detection level was increased to 8?10(4) PFU/ml A/Udorn/307/1972. Immuno-AuNPs were then complexed with viruses to permit direct observation, and they formed a ring of confined nanodots on the membrane of both intact and detergent-treated viruses as directly visualized by scanning electron microscopy. With this complex the detection limit was improved further to 8?10(3) PFU/ml on anti-rabbit IgG immobilized sensing plate. These strategies introduce methods for observing trapped intact viruses on the sensing plates generated for optical systems.
PMID:
23874887
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/23874887
Observations of immuno-gold conjugates on influenza viruses using waveguide-mode sensors.
Gopinath SC, Awazu K, Fujimaki M, Shimizu K, Shima T.
Source
Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Abstract
Gold nanoparticles were conjugated to an antibody (immuno-AuNP) against A/Udorn/307/1972 (H3N2) influenza virus to detect viruses on a sensing plate designed for an evanescent field-coupled waveguide-mode sensor. Experiments were conducted using human influenza A/H3N2 strains, and immuno-AuNP could detect 8?10(5) PFU/ml (40 pg/?l) intact A/Udorn/307/1972 and 120 pg/?l A/Brisbane/10/2007. Furthermore, increased signal magnitude was achieved in the presence of non-ionic detergent, as the virtual detection level was increased to 8?10(4) PFU/ml A/Udorn/307/1972. Immuno-AuNPs were then complexed with viruses to permit direct observation, and they formed a ring of confined nanodots on the membrane of both intact and detergent-treated viruses as directly visualized by scanning electron microscopy. With this complex the detection limit was improved further to 8?10(3) PFU/ml on anti-rabbit IgG immobilized sensing plate. These strategies introduce methods for observing trapped intact viruses on the sensing plates generated for optical systems.
PMID:
23874887
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/23874887