tetano
Editor, Senior Moderator
J Virol Methods. 2016 Mar 17. pii: S0166-0934(16)00053-7. doi: 10.1016/j.jviromet.2016.03.007. [Epub ahead of print]
[h=1]Optical fiber sensor based on surface plasmon resonance for rapid detection of avian influenza virus subtype H6: Initial studies.[/h] Zhao X[SUP]1[/SUP], Tsao YC[SUP]2[/SUP], Lee FJ[SUP]3[/SUP], Tsai WH[SUP]2[/SUP], Wang CH[SUP]4[/SUP], Chuang TL[SUP]3[/SUP], Wu MS[SUP]2[/SUP], Lin CW[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A side-polished fiber optic surface plasmon resonance (SPR) sensor was fabricated to expose the core surface and then deposited with a 40nm thin gold film for the near surface sensing of effective refractive index changes with surface concentration or thickness of captured avian influenza virus subtype H6. The detection surface of the SPR optical fiber sensor was prepared through the plasma modification method for binding a self-assembled monolayer of isopropanol chemically on the gold surface of the optical fiber. Subsequently, N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide/N-hydroxysuccinimide was activated to enable EB2-B3 monoclonal antibodies to capture A/chicken/Taiwan/2838V/00 (H6N1) through a flow injection system. The detection limit of the fabricated optical fiber sensor for A/chicken/Taiwan/2838V/00 was 5.14?10[SUP]5[/SUP] EID[SUB]50[/SUB]/0.1mL, and the response time was 10min on average. Moreover, the fiber optic sensor has the advantages of a compact size and low cost, thus rendering it suitable for online and remote sensing. The results indicated that the optical fiber sensor can be used for epidemiological surveillance and diagnosing of avian influenza subtype H6 rapidly.
Copyright ? 2016. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Avian influenza virus subtype H6; Optical fiber; Rapid detection; Surface plasmon resonance
PMID: 26996538 [PubMed - as supplied by publisher]
[h=1]Optical fiber sensor based on surface plasmon resonance for rapid detection of avian influenza virus subtype H6: Initial studies.[/h] Zhao X[SUP]1[/SUP], Tsao YC[SUP]2[/SUP], Lee FJ[SUP]3[/SUP], Tsai WH[SUP]2[/SUP], Wang CH[SUP]4[/SUP], Chuang TL[SUP]3[/SUP], Wu MS[SUP]2[/SUP], Lin CW[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A side-polished fiber optic surface plasmon resonance (SPR) sensor was fabricated to expose the core surface and then deposited with a 40nm thin gold film for the near surface sensing of effective refractive index changes with surface concentration or thickness of captured avian influenza virus subtype H6. The detection surface of the SPR optical fiber sensor was prepared through the plasma modification method for binding a self-assembled monolayer of isopropanol chemically on the gold surface of the optical fiber. Subsequently, N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide/N-hydroxysuccinimide was activated to enable EB2-B3 monoclonal antibodies to capture A/chicken/Taiwan/2838V/00 (H6N1) through a flow injection system. The detection limit of the fabricated optical fiber sensor for A/chicken/Taiwan/2838V/00 was 5.14?10[SUP]5[/SUP] EID[SUB]50[/SUB]/0.1mL, and the response time was 10min on average. Moreover, the fiber optic sensor has the advantages of a compact size and low cost, thus rendering it suitable for online and remote sensing. The results indicated that the optical fiber sensor can be used for epidemiological surveillance and diagnosing of avian influenza subtype H6 rapidly.
Copyright ? 2016. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Avian influenza virus subtype H6; Optical fiber; Rapid detection; Surface plasmon resonance
PMID: 26996538 [PubMed - as supplied by publisher]