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doi:10.1016/j.vaccine.2010.11.058 | How to Cite or Link Using DOI
Crown copyright ? 2010 Published by Elsevier Ltd.
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Optimal vaccination strategies for 2009 pandemic H1N1 and seasonal influenza vaccines in humansstar, open
, E-mail The Corresponding Author and Yuelong Shud, Corresponding Author Contact Information, E-mail The Corresponding Author
a Beijing Centre for Disease Control and Prevention, China
b Sinovac Biotech Co. Ltd, China
c MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, United Kingdom
d National Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, China
e Chaoyang District Centre for Disease Control and Prevention, China
f Department of Immunology, Capital Medical University, China
Received 17 June 2010;
revised 7 November 2010;
accepted 16 November 2010.
Available online 2 December 2010.
Abstract
A randomized clinical trial was conducted to assess whether the immunogenicity of seasonal and pandemic (H1N1/09) influenza vaccines is affected by the order of vaccine administration. 151 healthy adult volunteers were randomized into three groups. All groups received one dose (15 μg haemagglutinin) each of a pandemic H1N1 vaccine and a seasonal trivalent vaccine. Group 1 received the pandemic H1N1 vaccine first, followed by the seasonal vaccine 21 days later. Group 2 received vaccinations in vice versa and Group 3 received both vaccines simultaneously. Post-vaccination blood samples were collected to determine the immunogenicity by hemagglutination-inhibition (HI), microneutralization (MN), and B cell ELISPOT assays. All three vaccination strategies were well-tolerated and generated specific immune responses. However, we found a significant difference in magnitude of antibody responses to pandemic H1N1 between the three groups. Pre- or co-vaccination with the seasonal flu vaccine led to a significant reduction by 50% in HI titre to pandemic H1N1 virus after pandemic vaccination. Pre- or co-vaccination of pandemic H1N1 vaccine had no effect on seasonal flu vaccination. MN and ELISPOT assays showed a similar effect. Vaccination with pandemic H1N1 vaccine first is recommended to avoid an associated inhibitory effect by the seasonal trivalent flu vaccine. Clinical_Trials identifier: NCT01008137.
http://www.sciencedirect.com/scienc...dc66a8d15c8e413232aad9ed1db64cf1&searchtype=a
Crown copyright ? 2010 Published by Elsevier Ltd.
Permissions & Reprints
Optimal vaccination strategies for 2009 pandemic H1N1 and seasonal influenza vaccines in humansstar, open
, E-mail The Corresponding Author and Yuelong Shud, Corresponding Author Contact Information, E-mail The Corresponding Author
a Beijing Centre for Disease Control and Prevention, China
b Sinovac Biotech Co. Ltd, China
c MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, United Kingdom
d National Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, China
e Chaoyang District Centre for Disease Control and Prevention, China
f Department of Immunology, Capital Medical University, China
Received 17 June 2010;
revised 7 November 2010;
accepted 16 November 2010.
Available online 2 December 2010.
Abstract
A randomized clinical trial was conducted to assess whether the immunogenicity of seasonal and pandemic (H1N1/09) influenza vaccines is affected by the order of vaccine administration. 151 healthy adult volunteers were randomized into three groups. All groups received one dose (15 μg haemagglutinin) each of a pandemic H1N1 vaccine and a seasonal trivalent vaccine. Group 1 received the pandemic H1N1 vaccine first, followed by the seasonal vaccine 21 days later. Group 2 received vaccinations in vice versa and Group 3 received both vaccines simultaneously. Post-vaccination blood samples were collected to determine the immunogenicity by hemagglutination-inhibition (HI), microneutralization (MN), and B cell ELISPOT assays. All three vaccination strategies were well-tolerated and generated specific immune responses. However, we found a significant difference in magnitude of antibody responses to pandemic H1N1 between the three groups. Pre- or co-vaccination with the seasonal flu vaccine led to a significant reduction by 50% in HI titre to pandemic H1N1 virus after pandemic vaccination. Pre- or co-vaccination of pandemic H1N1 vaccine had no effect on seasonal flu vaccination. MN and ELISPOT assays showed a similar effect. Vaccination with pandemic H1N1 vaccine first is recommended to avoid an associated inhibitory effect by the seasonal trivalent flu vaccine. Clinical_Trials identifier: NCT01008137.
http://www.sciencedirect.com/scienc...dc66a8d15c8e413232aad9ed1db64cf1&searchtype=a