tetano
Editor, Senior Moderator
Oxid Med Cell Longev
. 2022 Jan 17;2022:5397733.
doi: 10.1155/2022/5397733. eCollection 2022.
Superoxide Dismutase Prevents SARS-CoV-2-Induced Plasma Cell Apoptosis and Stabilizes Specific Antibody Induction
Li-Hua Mo[SUP] 1 2 3 [/SUP], Xiang-Qian Luo[SUP] 1 [/SUP], Ke Ma[SUP] 4 [/SUP], Jian-Bo Shao[SUP] 1 5 6 [/SUP], Guo-Hao Zhang[SUP] 3 [/SUP], Zhi-Gang Liu[SUP] 3 [/SUP], Da-Bo Liu[SUP] 1 [/SUP], Huan-Ping Zhang[SUP] 4 [/SUP], Ping-Chang Yang[SUP] 2 3 [/SUP]
Affiliations
Abstract
The infection of coronavirus disease (COVID-19) seriously threatens human life. It is urgent to generate effective and safe specific antibodies (Abs) against the pathogenic elements of COVID-19. Mice were immunized with SARS-CoV-2 spike protein antigens: S ectodomain-1 (CoV, in short) mixed in Alum adjuvant for 2 times and boosted with CoV weekly for 6 times. A portion of mice were treated with Maotai liquor (MTL, in short) or/and heat stress (HS) together with CoV boosting. We observed that the anti-CoV Ab was successfully induced in mice that received the CoV/Alum immunization for 2 times. However, upon boosting with CoV, the CoV Ab production diminished progressively; spleen CoV Ab-producing plasma cell counts reduced, in which substantial CoV-specific Ab-producing plasma cells (sPC) were apoptotic. Apparent oxidative stress signs were observed in sPCs; the results were reproduced by exposing sPCs to CoV in the culture. The presence of MTL or/and HS prevented the CoV-induced oxidative stress in sPCs and promoted and stabilized the CoV Ab production in mice in re-exposure to CoV. In summary, CoV/Alum immunization can successfully induce CoV Ab production in mice that declines upon reexposure to CoV. Concurrent administration of MTL/HS stabilizes and promotes the CoV Ab production in mice.
. 2022 Jan 17;2022:5397733.
doi: 10.1155/2022/5397733. eCollection 2022.
Superoxide Dismutase Prevents SARS-CoV-2-Induced Plasma Cell Apoptosis and Stabilizes Specific Antibody Induction
Li-Hua Mo[SUP] 1 2 3 [/SUP], Xiang-Qian Luo[SUP] 1 [/SUP], Ke Ma[SUP] 4 [/SUP], Jian-Bo Shao[SUP] 1 5 6 [/SUP], Guo-Hao Zhang[SUP] 3 [/SUP], Zhi-Gang Liu[SUP] 3 [/SUP], Da-Bo Liu[SUP] 1 [/SUP], Huan-Ping Zhang[SUP] 4 [/SUP], Ping-Chang Yang[SUP] 2 3 [/SUP]
Affiliations
- PMID: 35047106
- PMCID: PMC8762758
- DOI: 10.1155/2022/5397733
Abstract
The infection of coronavirus disease (COVID-19) seriously threatens human life. It is urgent to generate effective and safe specific antibodies (Abs) against the pathogenic elements of COVID-19. Mice were immunized with SARS-CoV-2 spike protein antigens: S ectodomain-1 (CoV, in short) mixed in Alum adjuvant for 2 times and boosted with CoV weekly for 6 times. A portion of mice were treated with Maotai liquor (MTL, in short) or/and heat stress (HS) together with CoV boosting. We observed that the anti-CoV Ab was successfully induced in mice that received the CoV/Alum immunization for 2 times. However, upon boosting with CoV, the CoV Ab production diminished progressively; spleen CoV Ab-producing plasma cell counts reduced, in which substantial CoV-specific Ab-producing plasma cells (sPC) were apoptotic. Apparent oxidative stress signs were observed in sPCs; the results were reproduced by exposing sPCs to CoV in the culture. The presence of MTL or/and HS prevented the CoV-induced oxidative stress in sPCs and promoted and stabilized the CoV Ab production in mice in re-exposure to CoV. In summary, CoV/Alum immunization can successfully induce CoV Ab production in mice that declines upon reexposure to CoV. Concurrent administration of MTL/HS stabilizes and promotes the CoV Ab production in mice.