tetano
Editor, Senior Moderator
J Gen Virol. 2017 Jan 18. doi: 10.1099/jgv.0.000715. [Epub ahead of print]
[h=1]Pathogenicity of modified bat influenza virus with different M genes and its reassortment potential with swine influenza A virus.[/h] Yang J[SUP]1[/SUP], Lee J[SUP]2[/SUP], Ma J[SUP]3[/SUP], Lang Y[SUP]4[/SUP], Nietfeld J[SUP]5[/SUP], Li Y[SUP]6[/SUP], Duff M[SUP]7[/SUP], Li Y[SUP]8[/SUP], Yang Y[SUP]9[/SUP], Liu H[SUP]10[/SUP], Zhou B[SUP]11[/SUP], Wentworth DE[SUP]12[/SUP], Richt JA[SUP]13[/SUP], Li Z[SUP]14[/SUP], Ma W[SUP]15[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In our previous studies the reassortant virus containing only the PR8 H1N1 matrix (M) gene in the background of the modified bat influenza Bat09:mH1mN1 virus could be generated. However, whether M genes from other origins can be rescued in the background of the Bat09:mH1mN1 virus and whether the resulting novel reassortant virus is virulent remain unknown. Herein, two reassortant viruses were generated in the background of the Bat09:mH1mN1 virus containing either a North American or a Eurasian swine influenza virus M gene. These two reassortant viruses and the reassortant virus with PR8 M as well as the control Bat09:mH1mN1 virus replicated efficiently in cultured cells, while the reassortant virus with PR8 M grew to a higher titer than the other three viruses in tested cells. Mouse studies showed that reassortant viruses with either North American or Eurasian swine influenza virus M genes did not enhance virulence, whereas the reassortant virus with PR8 M gene displayed higher pathogenicity when compared to the Bat09:mH1mN1 virus. This is most likely due to the fact that the PR8 H1N1 virus is a mouse-adapted virus. Furthermore, reassortment potential between the Bat09:mH1mN1 virus and an H3N2 swine influenza virus (A/swine/Texas/4199-2/1998) was investigated using co-infection of MDCK cells, but no reassortant viruses were detected. Taken together, our results indicate that the modified bat influenza virus is most likely incapable of reassortment with influenza A viruses with in vitro co-infection experiments, although reassortant viruses with different M genes can be generated by reverse genetics.
PMID: 28100299 DOI: 10.1099/jgv.0.000715
[PubMed - as supplied by publisher]
[h=1]Pathogenicity of modified bat influenza virus with different M genes and its reassortment potential with swine influenza A virus.[/h] Yang J[SUP]1[/SUP], Lee J[SUP]2[/SUP], Ma J[SUP]3[/SUP], Lang Y[SUP]4[/SUP], Nietfeld J[SUP]5[/SUP], Li Y[SUP]6[/SUP], Duff M[SUP]7[/SUP], Li Y[SUP]8[/SUP], Yang Y[SUP]9[/SUP], Liu H[SUP]10[/SUP], Zhou B[SUP]11[/SUP], Wentworth DE[SUP]12[/SUP], Richt JA[SUP]13[/SUP], Li Z[SUP]14[/SUP], Ma W[SUP]15[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In our previous studies the reassortant virus containing only the PR8 H1N1 matrix (M) gene in the background of the modified bat influenza Bat09:mH1mN1 virus could be generated. However, whether M genes from other origins can be rescued in the background of the Bat09:mH1mN1 virus and whether the resulting novel reassortant virus is virulent remain unknown. Herein, two reassortant viruses were generated in the background of the Bat09:mH1mN1 virus containing either a North American or a Eurasian swine influenza virus M gene. These two reassortant viruses and the reassortant virus with PR8 M as well as the control Bat09:mH1mN1 virus replicated efficiently in cultured cells, while the reassortant virus with PR8 M grew to a higher titer than the other three viruses in tested cells. Mouse studies showed that reassortant viruses with either North American or Eurasian swine influenza virus M genes did not enhance virulence, whereas the reassortant virus with PR8 M gene displayed higher pathogenicity when compared to the Bat09:mH1mN1 virus. This is most likely due to the fact that the PR8 H1N1 virus is a mouse-adapted virus. Furthermore, reassortment potential between the Bat09:mH1mN1 virus and an H3N2 swine influenza virus (A/swine/Texas/4199-2/1998) was investigated using co-infection of MDCK cells, but no reassortant viruses were detected. Taken together, our results indicate that the modified bat influenza virus is most likely incapable of reassortment with influenza A viruses with in vitro co-infection experiments, although reassortant viruses with different M genes can be generated by reverse genetics.
PMID: 28100299 DOI: 10.1099/jgv.0.000715
[PubMed - as supplied by publisher]