tetano
Editor, Senior Moderator
Pathogens
. 2021 Jun 23;10(7):798.
doi: 10.3390/pathogens10070798.
Occurrence of SARS-CoV-2 RNA in Six Municipal Wastewater Treatment Plants at the Early Stage of COVID-19 Pandemic in The United States
Samendra P Sherchan[SUP] 1 [/SUP], Shalina Shahin[SUP] 1 [/SUP], Jeenal Patel[SUP] 1 [/SUP], Lauren M Ward[SUP] 1 [/SUP], Sarmila Tandukar[SUP] 2 [/SUP], Sital Uprety[SUP] 3 [/SUP], Bradley W Schmitz[SUP] 4 [/SUP], Warish Ahmed[SUP] 5 [/SUP], Stuart Simpson[SUP] 6 [/SUP], Pradip Gyawali[SUP] 7 [/SUP]
Affiliations
Abstract
In this study, we investigated the occurrence of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) RNA in primary influent (n = 42), secondary effluent (n = 24) and tertiary treated effluent (n = 34) collected from six wastewater treatment plants (WWTPs A-F) in Virginia (WWTP A), Florida (WWTPs B, C, and D), and Georgia (WWTPs E and F) in the United States during April-July 2020. Of the 100 wastewater samples analyzed, eight (19%) untreated wastewater samples collected from the primary influents contained SARS-CoV-2 RNA as measured by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays. SARS-CoV-2 RNA were detected in influent wastewater samples collected from WWTP A (Virginia), WWTPs E and F (Georgia) and WWTP D (Florida). Secondary and tertiary effluent samples were not positive for SARS-CoV-2 RNA indicating the treatment processes in these WWTPs potentially removed SARS-CoV-2 RNA during the secondary and tertiary treatment processes. However, further studies are needed to understand the log removal values (LRVs) and transmission risks of SARS-CoV-2 RNA through analyzing wastewater samples from a wider range of WWTPs.
Keywords: COVID-19; SARS-CoV-2 RNA; transmission risk; wastewater; wastewater treatment.
. 2021 Jun 23;10(7):798.
doi: 10.3390/pathogens10070798.
Occurrence of SARS-CoV-2 RNA in Six Municipal Wastewater Treatment Plants at the Early Stage of COVID-19 Pandemic in The United States
Samendra P Sherchan[SUP] 1 [/SUP], Shalina Shahin[SUP] 1 [/SUP], Jeenal Patel[SUP] 1 [/SUP], Lauren M Ward[SUP] 1 [/SUP], Sarmila Tandukar[SUP] 2 [/SUP], Sital Uprety[SUP] 3 [/SUP], Bradley W Schmitz[SUP] 4 [/SUP], Warish Ahmed[SUP] 5 [/SUP], Stuart Simpson[SUP] 6 [/SUP], Pradip Gyawali[SUP] 7 [/SUP]
Affiliations
- PMID: 34201687
- DOI: 10.3390/pathogens10070798
Abstract
In this study, we investigated the occurrence of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) RNA in primary influent (n = 42), secondary effluent (n = 24) and tertiary treated effluent (n = 34) collected from six wastewater treatment plants (WWTPs A-F) in Virginia (WWTP A), Florida (WWTPs B, C, and D), and Georgia (WWTPs E and F) in the United States during April-July 2020. Of the 100 wastewater samples analyzed, eight (19%) untreated wastewater samples collected from the primary influents contained SARS-CoV-2 RNA as measured by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays. SARS-CoV-2 RNA were detected in influent wastewater samples collected from WWTP A (Virginia), WWTPs E and F (Georgia) and WWTP D (Florida). Secondary and tertiary effluent samples were not positive for SARS-CoV-2 RNA indicating the treatment processes in these WWTPs potentially removed SARS-CoV-2 RNA during the secondary and tertiary treatment processes. However, further studies are needed to understand the log removal values (LRVs) and transmission risks of SARS-CoV-2 RNA through analyzing wastewater samples from a wider range of WWTPs.
Keywords: COVID-19; SARS-CoV-2 RNA; transmission risk; wastewater; wastewater treatment.