tetano
Editor, Senior Moderator
J Clin Virol. 2015 Sep;70:72-6. doi: 10.1016/j.jcv.2015.07.294. Epub 2015 Jul 17.
[h=1]Performance of the Alere i influenza A&B assay and mariPOC test for the rapid detection of influenza A and B viruses.[/h] Jokela P[SUP]1[/SUP], Vuorinen T[SUP]2[/SUP], Waris M[SUP]2[/SUP], Manninen R[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Timely detection of influenza viruses is required to facilitate infection control measures and appropriate patient management. The Alere? i Influenza A&B assay for detection of viral RNA and multianalyte mariPOC(?) test for detection of viral antigens enable rapid detection of influenza viruses with little hands-on time.
[h=4]OBJECTIVES:[/h] To evaluate the performance of the Alere i Influenza A&B assay and the mariPOC test in comparison to the Xpert(?) Flu A/B assay and laboratory-developed real-time reverse transcription-polymerase chain reaction.
[h=4]STUDY DESIGN:[/h] A total of 140 and 108 nasopharyngeal specimens were analysed for evaluation of the Alere i and mariPOC, respectively.
[h=4]RESULTS:[/h] The sensitivity and specificity of the Alere i Influenza A&B assay for detection of influenza A virus was 80.0% and 98.1%, and for influenza B virus 45.2% and 98.2%, respectively. For the mariPOC test, a sensitivity and specificity of 53.1% and 98.7%, respectively, for detection of influenza A virus was achieved.
[h=4]CONCLUSIONS:[/h] The mariPOC test proved insensitive for detection of influenza A virus and therefore unsuitable for individual patient diagnosis without confirmatory testing. In contrast, the Alere i Influenza A&B assay was reasonably sensitive and specific for detection of influenza A and B virus, although decreased detection of specimens with low viral load was observed particularly for detection of influenza B virus. Taken together with its rapidity and ease of use, the Alere i influenza A&B assay is a welcome alternative to immunochromatographic rapid influenza detection tests and may provide timely results that enable appropriate patient care and management of patient flow during high-prevalence seasons.
Copyright ? 2015 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Alere i; Influenza; POC; Rapid antigen detection; Real-time RT-PCR; mariPOC
PMID: 26305824 [PubMed - in process]
[h=1]Performance of the Alere i influenza A&B assay and mariPOC test for the rapid detection of influenza A and B viruses.[/h] Jokela P[SUP]1[/SUP], Vuorinen T[SUP]2[/SUP], Waris M[SUP]2[/SUP], Manninen R[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Timely detection of influenza viruses is required to facilitate infection control measures and appropriate patient management. The Alere? i Influenza A&B assay for detection of viral RNA and multianalyte mariPOC(?) test for detection of viral antigens enable rapid detection of influenza viruses with little hands-on time.
[h=4]OBJECTIVES:[/h] To evaluate the performance of the Alere i Influenza A&B assay and the mariPOC test in comparison to the Xpert(?) Flu A/B assay and laboratory-developed real-time reverse transcription-polymerase chain reaction.
[h=4]STUDY DESIGN:[/h] A total of 140 and 108 nasopharyngeal specimens were analysed for evaluation of the Alere i and mariPOC, respectively.
[h=4]RESULTS:[/h] The sensitivity and specificity of the Alere i Influenza A&B assay for detection of influenza A virus was 80.0% and 98.1%, and for influenza B virus 45.2% and 98.2%, respectively. For the mariPOC test, a sensitivity and specificity of 53.1% and 98.7%, respectively, for detection of influenza A virus was achieved.
[h=4]CONCLUSIONS:[/h] The mariPOC test proved insensitive for detection of influenza A virus and therefore unsuitable for individual patient diagnosis without confirmatory testing. In contrast, the Alere i Influenza A&B assay was reasonably sensitive and specific for detection of influenza A and B virus, although decreased detection of specimens with low viral load was observed particularly for detection of influenza B virus. Taken together with its rapidity and ease of use, the Alere i influenza A&B assay is a welcome alternative to immunochromatographic rapid influenza detection tests and may provide timely results that enable appropriate patient care and management of patient flow during high-prevalence seasons.
Copyright ? 2015 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Alere i; Influenza; POC; Rapid antigen detection; Real-time RT-PCR; mariPOC
PMID: 26305824 [PubMed - in process]