tetano
Editor, Senior Moderator
J Infect Dis. 2012 Mar 28. [Epub ahead of print]
Phenotypic and functional characterization of human γδ T cell subsets in response to influenza A viruses.
Qin G, Liu Y, Zheng J, Xiang Z, Ng IH, Peiris JS, Lau YL, Tu W.
Source
Department of Paediatrics & Adolescent Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong SAR, China.
Abstract
Like αβ T cells, human γδ T cells also have different subsets with distinct characteristics. Whether human Vγ9Vδ2 T cells have functionally different subsets in response to influenza A (fluA) viruses remains unknown. In this study, we have shown for the first time that both central (CD45RA(-)CD27(+)) and effector (CD45RA(-)CD27(-)) memory Vγ9Vδ2 T had similar levels of immediate IFN-γ and cytotoxic responses to human and avian fluA virus-infected cells. In contrast, CD56(+) Vγ9Vδ2 T cells had significantly higher cytotoxicity against fluA virus-infected cells, compared with CD56(-) counterparts, while both subsets had similar IFN-γ responses. We further demonstrated that the CD16-dependant degranulation pathway, but not antibody-dependent cell-mediated cytotoxicity (ADCC), contributed to the superior cytotoxicity of CD56(+) Vγ9Vδ2 T cells. Our study provides further evidence for the phenotypic and functional characterization of human Vγ9Vδ2 T subsets during fluA virus infection, and may help improve the γδ T cell-based immunotherapy for viral infection.
PMID:
22457284
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22457284
Phenotypic and functional characterization of human γδ T cell subsets in response to influenza A viruses.
Qin G, Liu Y, Zheng J, Xiang Z, Ng IH, Peiris JS, Lau YL, Tu W.
Source
Department of Paediatrics & Adolescent Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong SAR, China.
Abstract
Like αβ T cells, human γδ T cells also have different subsets with distinct characteristics. Whether human Vγ9Vδ2 T cells have functionally different subsets in response to influenza A (fluA) viruses remains unknown. In this study, we have shown for the first time that both central (CD45RA(-)CD27(+)) and effector (CD45RA(-)CD27(-)) memory Vγ9Vδ2 T had similar levels of immediate IFN-γ and cytotoxic responses to human and avian fluA virus-infected cells. In contrast, CD56(+) Vγ9Vδ2 T cells had significantly higher cytotoxicity against fluA virus-infected cells, compared with CD56(-) counterparts, while both subsets had similar IFN-γ responses. We further demonstrated that the CD16-dependant degranulation pathway, but not antibody-dependent cell-mediated cytotoxicity (ADCC), contributed to the superior cytotoxicity of CD56(+) Vγ9Vδ2 T cells. Our study provides further evidence for the phenotypic and functional characterization of human Vγ9Vδ2 T subsets during fluA virus infection, and may help improve the γδ T cell-based immunotherapy for viral infection.
PMID:
22457284
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22457284