tetano
Editor, Senior Moderator
Arch Virol. 2017 Jul 3. doi: 10.1007/s00705-017-3474-2. [Epub ahead of print]
[h=1]Phylogenetic analysis of H9N2 avian influenza viruses in Afghanistan (2016-2017).[/h] Hosseini H[SUP]1[/SUP], Ghalyanchilangeroudi A[SUP]2[/SUP], Fallah Mehrabadi MH[SUP]3[/SUP], Sediqian MS[SUP]4[/SUP], Shayeganmehr A[SUP]5[/SUP], Ghafouri SA[SUP]6[/SUP], Maghsoudloo H[SUP]6[/SUP], Abdollahi H[SUP]6[/SUP], Farahani RK[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Avian influenza A virus (AIV) subtype H9N2 is the most prevalent subtype found in terrestrial poultry throughout Eurasia and has been isolated from poultry outbreaks worldwide. Tracheal tissue specimens from 100 commercial broiler flocks in Afghanistan were collected between 2016 and 2017. After real-time RT-PCR, AI-positive samples were further characterized. A part of the HA gene was amplified using RT-PCR and sequenced. The results of real-time RT-PCR showed that 40 percent of the flocks were AI positive. Phylogenetic studies showed that these H9N2 AIVs grouped within the Eurasian-lineage G1 AIVs and had a correlation with H9N2 AIV circulating in the poultry population of the neighboring countries over the past decade. Analysis of the amino acid sequence of HA revealed that the detected H9N2 viruses possessed molecular profiles suggestive of low pathogenicity and specificity for the avian-like SAα2,3 receptor, demonstrating their specificity for and adaptation to domestic poultry. The results of the current study provide great insights into H9N2 viruses circulating in Afghanistan's poultry industry and demonstrate the necessity of planning an applied policy aimed at controlling and managing H9N2 infection in Afghan poultry.
[h=4]KEYWORDS:[/h] Afghanistan; Avian influenza; H9N2; Phylogenetic study
PMID: 28674864 DOI: 10.1007/s00705-017-3474-2
[h=1]Phylogenetic analysis of H9N2 avian influenza viruses in Afghanistan (2016-2017).[/h] Hosseini H[SUP]1[/SUP], Ghalyanchilangeroudi A[SUP]2[/SUP], Fallah Mehrabadi MH[SUP]3[/SUP], Sediqian MS[SUP]4[/SUP], Shayeganmehr A[SUP]5[/SUP], Ghafouri SA[SUP]6[/SUP], Maghsoudloo H[SUP]6[/SUP], Abdollahi H[SUP]6[/SUP], Farahani RK[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Avian influenza A virus (AIV) subtype H9N2 is the most prevalent subtype found in terrestrial poultry throughout Eurasia and has been isolated from poultry outbreaks worldwide. Tracheal tissue specimens from 100 commercial broiler flocks in Afghanistan were collected between 2016 and 2017. After real-time RT-PCR, AI-positive samples were further characterized. A part of the HA gene was amplified using RT-PCR and sequenced. The results of real-time RT-PCR showed that 40 percent of the flocks were AI positive. Phylogenetic studies showed that these H9N2 AIVs grouped within the Eurasian-lineage G1 AIVs and had a correlation with H9N2 AIV circulating in the poultry population of the neighboring countries over the past decade. Analysis of the amino acid sequence of HA revealed that the detected H9N2 viruses possessed molecular profiles suggestive of low pathogenicity and specificity for the avian-like SAα2,3 receptor, demonstrating their specificity for and adaptation to domestic poultry. The results of the current study provide great insights into H9N2 viruses circulating in Afghanistan's poultry industry and demonstrate the necessity of planning an applied policy aimed at controlling and managing H9N2 infection in Afghan poultry.
[h=4]KEYWORDS:[/h] Afghanistan; Avian influenza; H9N2; Phylogenetic study
PMID: 28674864 DOI: 10.1007/s00705-017-3474-2