tetano
Editor, Senior Moderator
PLoS One
. 2020 Oct 22;15(10):e0240395.
doi: 10.1371/journal.pone.0240395. eCollection 2020.
Antibody kinetics and serologic profiles of SARS-CoV-2 infection using two serologic assays
Oh Joo Kweon[SUP] 1 [/SUP], Yong Kwan Lim[SUP] 1 [/SUP], Hye Ryoun Kim[SUP] 1 [/SUP], Min-Chul Kim[SUP] 2 [/SUP], Seong-Ho Choi[SUP] 2 [/SUP], Jin-Won Chung[SUP] 2 [/SUP], Mi-Kyung Lee[SUP] 1 [/SUP]
Affiliations
Abstract
Background: Coronavirus disease 2019 (COVID-19) is an emerging threat worldwide. This study aims to assess the serologic profiles and time kinetics of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in patients with COVID-19 using two immunoassays.
Methods: A total of 97 samples serially collected from 17 patients with COVID-19 and 137 negative control samples were analyzed for IgM and IgG against SARS-CoV-2 using the AFIAS COVID-19 Ab (Boditech Med Inc., Chuncheon, Republic of Korea) and the EDI™ Novel Coronavirus COVID-19 ELISA Kit (Epitope Diagnostics, Inc., San Diego, CA).
Results: With both assays, IgM and IgG rapidly increased after 7 days post symptom onset (PSO). IgM antibody levels reached a peak at 15-35 d PSO and gradually decreased. IgG levels gradually increased and remained at similar levels after 22-35 d. The diagnostic sensitivities of IgM/IgG for ≤14d PSO were 21.4%/35.7~57.1% and increased to 41.2~52.9%/88.2~94.1% at >14 d PSO with specificities of 98.5%/94.2% for AFIAS COVID-19 Ab and 100.0%/96.4% for EDI™ Novel Coronavirus COVID-19 ELISA Kit. Among 137 negative controls, 12 samples (8.8%) showed positive or indeterminate results.
Conclusions: The antibody kinetics against SARS-CoV-2 are similar to common findings of acute viral infectious diseases. Antibody testing is useful for ruling out SARS-CoV-2 infection after 14 d PSO, detecting past infection, and epidemiologic surveys.
. 2020 Oct 22;15(10):e0240395.
doi: 10.1371/journal.pone.0240395. eCollection 2020.
Antibody kinetics and serologic profiles of SARS-CoV-2 infection using two serologic assays
Oh Joo Kweon[SUP] 1 [/SUP], Yong Kwan Lim[SUP] 1 [/SUP], Hye Ryoun Kim[SUP] 1 [/SUP], Min-Chul Kim[SUP] 2 [/SUP], Seong-Ho Choi[SUP] 2 [/SUP], Jin-Won Chung[SUP] 2 [/SUP], Mi-Kyung Lee[SUP] 1 [/SUP]
Affiliations
- PMID: 33091042
- DOI: 10.1371/journal.pone.0240395
Abstract
Background: Coronavirus disease 2019 (COVID-19) is an emerging threat worldwide. This study aims to assess the serologic profiles and time kinetics of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in patients with COVID-19 using two immunoassays.
Methods: A total of 97 samples serially collected from 17 patients with COVID-19 and 137 negative control samples were analyzed for IgM and IgG against SARS-CoV-2 using the AFIAS COVID-19 Ab (Boditech Med Inc., Chuncheon, Republic of Korea) and the EDI™ Novel Coronavirus COVID-19 ELISA Kit (Epitope Diagnostics, Inc., San Diego, CA).
Results: With both assays, IgM and IgG rapidly increased after 7 days post symptom onset (PSO). IgM antibody levels reached a peak at 15-35 d PSO and gradually decreased. IgG levels gradually increased and remained at similar levels after 22-35 d. The diagnostic sensitivities of IgM/IgG for ≤14d PSO were 21.4%/35.7~57.1% and increased to 41.2~52.9%/88.2~94.1% at >14 d PSO with specificities of 98.5%/94.2% for AFIAS COVID-19 Ab and 100.0%/96.4% for EDI™ Novel Coronavirus COVID-19 ELISA Kit. Among 137 negative controls, 12 samples (8.8%) showed positive or indeterminate results.
Conclusions: The antibody kinetics against SARS-CoV-2 are similar to common findings of acute viral infectious diseases. Antibody testing is useful for ruling out SARS-CoV-2 infection after 14 d PSO, detecting past infection, and epidemiologic surveys.