tetano
Editor, Senior Moderator
PLoS One
. 2022 May 10;17(5):e0268279.
doi: 10.1371/journal.pone.0268279. eCollection 2022.
Assessment of potential factors associated with the sensitivity and specificity of Sofia Influenza A+B Fluorescent Immunoassay in an ambulatory care setting
Cristalyne Bell[SUP] 1 [/SUP], Maureen Goss[SUP] 1 [/SUP], Jennifer Birstler[SUP] 2 [/SUP], Emily Temte[SUP] 1 [/SUP], Guanhua Chen[SUP] 2 [/SUP], Peter Shult[SUP] 3 [/SUP], Erik Reisdorf[SUP] 3 [/SUP], Thomas Haupt[SUP] 4 [/SUP], Shari Barlow[SUP] 1 [/SUP], Jonathan Temte[SUP] 1 [/SUP]
Affiliations
Abstract
Background: Seasonal influenza leads to an increase in outpatient clinic visits. Timely, accurate, and affordable testing could facilitate improved treatment outcomes. Rapid influenza diagnostic tests (RIDTs) provide results in as little as 15 minutes and are relatively inexpensive, but have reduced sensitivity when compared to RT-PCR. The contributions of multiple factors related to test performance are not well defined for ambulatory care settings. We assessed clinical and laboratory factors that may affect the sensitivity and specificity of Sofia Influenza A+B Fluorescence Immunoassay.
Study design: We performed a post-hoc assessment of surveillance data amassed over seven years from five primary care clinics. We analyzed 4,475 paired RIDT and RT-PCR results from specimens collected from patients presenting with respiratory symptoms and examined eleven potential factors with additional sub-categories that could affect RIDT sensitivity.
Results: In an unadjusted analysis, greater sensitivity was associated with the presence of an influenza-like illness (ILI), no other virus detected, no seasonal influenza vaccination, younger age, lower cycle threshold value, fewer days since illness onset, nasal discharge, stuffy nose, and fever. After adjustment, presence of an ILI, younger age, fewer days from onset, no co-detection, and presence of a nasal discharge maintained significance.
Conclusion: Clinical and laboratory factors may affect RIDT sensitivity. Identifying potential factors during point-of-care testing could aid clinicians in appropriately interpreting negative influenza RIDT results.
. 2022 May 10;17(5):e0268279.
doi: 10.1371/journal.pone.0268279. eCollection 2022.
Assessment of potential factors associated with the sensitivity and specificity of Sofia Influenza A+B Fluorescent Immunoassay in an ambulatory care setting
Cristalyne Bell[SUP] 1 [/SUP], Maureen Goss[SUP] 1 [/SUP], Jennifer Birstler[SUP] 2 [/SUP], Emily Temte[SUP] 1 [/SUP], Guanhua Chen[SUP] 2 [/SUP], Peter Shult[SUP] 3 [/SUP], Erik Reisdorf[SUP] 3 [/SUP], Thomas Haupt[SUP] 4 [/SUP], Shari Barlow[SUP] 1 [/SUP], Jonathan Temte[SUP] 1 [/SUP]
Affiliations
- PMID: 35536787
- DOI: 10.1371/journal.pone.0268279
Abstract
Background: Seasonal influenza leads to an increase in outpatient clinic visits. Timely, accurate, and affordable testing could facilitate improved treatment outcomes. Rapid influenza diagnostic tests (RIDTs) provide results in as little as 15 minutes and are relatively inexpensive, but have reduced sensitivity when compared to RT-PCR. The contributions of multiple factors related to test performance are not well defined for ambulatory care settings. We assessed clinical and laboratory factors that may affect the sensitivity and specificity of Sofia Influenza A+B Fluorescence Immunoassay.
Study design: We performed a post-hoc assessment of surveillance data amassed over seven years from five primary care clinics. We analyzed 4,475 paired RIDT and RT-PCR results from specimens collected from patients presenting with respiratory symptoms and examined eleven potential factors with additional sub-categories that could affect RIDT sensitivity.
Results: In an unadjusted analysis, greater sensitivity was associated with the presence of an influenza-like illness (ILI), no other virus detected, no seasonal influenza vaccination, younger age, lower cycle threshold value, fewer days since illness onset, nasal discharge, stuffy nose, and fever. After adjustment, presence of an ILI, younger age, fewer days from onset, no co-detection, and presence of a nasal discharge maintained significance.
Conclusion: Clinical and laboratory factors may affect RIDT sensitivity. Identifying potential factors during point-of-care testing could aid clinicians in appropriately interpreting negative influenza RIDT results.