tetano
Editor, Senior Moderator
PLoS One
. 2021 Jun 10;16(6):e0252964.
doi: 10.1371/journal.pone.0252964. eCollection 2021.
Detection of SARS-CoV-2 using qRT-PCR in saliva obtained from asymptomatic or mild COVID-19 patients, comparative analysis with matched nasopharyngeal samples
Kenji Ota[SUP] 1 [/SUP], Katsunori Yanagihara[SUP] 1 [/SUP], Daisuke Sasaki[SUP] 1 [/SUP], Norihito Kaku[SUP] 1 [/SUP], Naoki Uno[SUP] 1 [/SUP], Kei Sakamoto[SUP] 1 [/SUP], Kosuke Kosai[SUP] 1 [/SUP], Taiga Miyazaki[SUP] 2 [/SUP], Hiroo Hasegawa[SUP] 1 [/SUP], Ayumi Fujita[SUP] 3 [/SUP], Masato Tashiro[SUP] 3 [/SUP], Takeshi Tanaka[SUP] 3 [/SUP], Koichi Izumikawa[SUP] 3 [/SUP], Koya Ariyoshi[SUP] 4 [/SUP], Hiroshi Mukae[SUP] 2 [/SUP], Jiro Yasuda[SUP] 5 [/SUP], Kouichi Morita[SUP] 5 [/SUP], Shigeru Kohno[SUP] 6 [/SUP]
Affiliations
Abstract
Objectives: The accurate detection of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is essential for the diagnosis of coronavirus disease 2019 (COVID-19). We compared the quantitative RT-PCR results between nasopharyngeal swabs and saliva specimens.
Methods: A COVID-19 outbreak occurred on a cruise ship at Nagasaki port, Japan. We obtained 123 nasopharyngeal swabs and saliva each from asymptomatic or mild patients in the late phase of infection.
Results: The intervals from the diagnosis to the sampling were 25.5 days for nasopharyngeal swabs and 28.9 days for saliva. The positive rate was 19.5% (24/123) for nasopharyngeal swabs and 38.2% (47/123) for saliva (P = 0.48). The quantified viral copies (mean ± SEM copies/5 μl) were 9.3±2.6 in nasopharyngeal swabs and 920±850 in saliva (P = 0.0006).
Conclusions: The advantages of saliva specimens include positive rate improvement and accurate viral load detection. Saliva may be used as a reliable sample for SARS-CoV-2 detection.
. 2021 Jun 10;16(6):e0252964.
doi: 10.1371/journal.pone.0252964. eCollection 2021.
Detection of SARS-CoV-2 using qRT-PCR in saliva obtained from asymptomatic or mild COVID-19 patients, comparative analysis with matched nasopharyngeal samples
Kenji Ota[SUP] 1 [/SUP], Katsunori Yanagihara[SUP] 1 [/SUP], Daisuke Sasaki[SUP] 1 [/SUP], Norihito Kaku[SUP] 1 [/SUP], Naoki Uno[SUP] 1 [/SUP], Kei Sakamoto[SUP] 1 [/SUP], Kosuke Kosai[SUP] 1 [/SUP], Taiga Miyazaki[SUP] 2 [/SUP], Hiroo Hasegawa[SUP] 1 [/SUP], Ayumi Fujita[SUP] 3 [/SUP], Masato Tashiro[SUP] 3 [/SUP], Takeshi Tanaka[SUP] 3 [/SUP], Koichi Izumikawa[SUP] 3 [/SUP], Koya Ariyoshi[SUP] 4 [/SUP], Hiroshi Mukae[SUP] 2 [/SUP], Jiro Yasuda[SUP] 5 [/SUP], Kouichi Morita[SUP] 5 [/SUP], Shigeru Kohno[SUP] 6 [/SUP]
Affiliations
- PMID: 34111203
- DOI: 10.1371/journal.pone.0252964
Abstract
Objectives: The accurate detection of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) is essential for the diagnosis of coronavirus disease 2019 (COVID-19). We compared the quantitative RT-PCR results between nasopharyngeal swabs and saliva specimens.
Methods: A COVID-19 outbreak occurred on a cruise ship at Nagasaki port, Japan. We obtained 123 nasopharyngeal swabs and saliva each from asymptomatic or mild patients in the late phase of infection.
Results: The intervals from the diagnosis to the sampling were 25.5 days for nasopharyngeal swabs and 28.9 days for saliva. The positive rate was 19.5% (24/123) for nasopharyngeal swabs and 38.2% (47/123) for saliva (P = 0.48). The quantified viral copies (mean ± SEM copies/5 μl) were 9.3±2.6 in nasopharyngeal swabs and 920±850 in saliva (P = 0.0006).
Conclusions: The advantages of saliva specimens include positive rate improvement and accurate viral load detection. Saliva may be used as a reliable sample for SARS-CoV-2 detection.