tetano
Editor, Senior Moderator
PLoS One
. 2020 Oct 20;15(10):e0240012.
doi: 10.1371/journal.pone.0240012. eCollection 2020.
Proteins associated with neutrophil degranulation are upregulated in nasopharyngeal swabs from SARS-CoV-2 patients
Emel Akgun[SUP] 1 2 [/SUP], Mete Bora Tuzuner[SUP] 2 [/SUP], Betul Sahin[SUP] 2 [/SUP], Meltem Kilercik[SUP] 1 2 [/SUP], Canan Kulah[SUP] 2 [/SUP], Hacer Nur Cakiroglu[SUP] 2 [/SUP], Mustafa Serteser[SUP] 1 2 [/SUP], Ibrahim Unsal[SUP] 2 [/SUP], Ahmet Tarik Baykal[SUP] 1 2 [/SUP]
Affiliations
Abstract
COVID-19 or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) appeared throughout the World and currently affected more than 9 million people and caused the death of around 470,000 patients. The novel strain of the coronavirus disease is transmittable at a devastating rate with a high rate of severe hospitalization even more so for the elderly population. Naso-oro-pharyngeal swab samples as the first step towards detecting suspected infection of SARS-CoV-2 provides a non-invasive method for PCR testing at a high confidence rate. Furthermore, proteomics analysis of PCR positive and negative naso-oropharyngeal samples provides information on the molecular level which highlights disease pathology. Samples from 15 PCR positive cases and 15 PCR negative cases were analyzed with nanoLC-MS/MS to identify the differentially expressed proteins. Proteomic analyses identified 207 proteins across the sample set and 17 of them were statistically significant. Protein-protein interaction analyses emphasized pathways like Neutrophil degranulation, Innate Immune System, Antimicrobial Peptides. Neutrophil Elastase (ELANE), Azurocidin (AZU1), Myeloperoxidase (MPO), Myeloblastin (PRTN3), Cathepsin G (CTSG) and Transcobalamine-1 (TCN1) were found to be significantly altered in naso-oropharyngeal samples of SARS-CoV-2 patients. The identified proteins are linked to alteration in the innate immune system specifically via neutrophil degranulation and NETosis.
. 2020 Oct 20;15(10):e0240012.
doi: 10.1371/journal.pone.0240012. eCollection 2020.
Proteins associated with neutrophil degranulation are upregulated in nasopharyngeal swabs from SARS-CoV-2 patients
Emel Akgun[SUP] 1 2 [/SUP], Mete Bora Tuzuner[SUP] 2 [/SUP], Betul Sahin[SUP] 2 [/SUP], Meltem Kilercik[SUP] 1 2 [/SUP], Canan Kulah[SUP] 2 [/SUP], Hacer Nur Cakiroglu[SUP] 2 [/SUP], Mustafa Serteser[SUP] 1 2 [/SUP], Ibrahim Unsal[SUP] 2 [/SUP], Ahmet Tarik Baykal[SUP] 1 2 [/SUP]
Affiliations
- PMID: 33079950
- DOI: 10.1371/journal.pone.0240012
Abstract
COVID-19 or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) appeared throughout the World and currently affected more than 9 million people and caused the death of around 470,000 patients. The novel strain of the coronavirus disease is transmittable at a devastating rate with a high rate of severe hospitalization even more so for the elderly population. Naso-oro-pharyngeal swab samples as the first step towards detecting suspected infection of SARS-CoV-2 provides a non-invasive method for PCR testing at a high confidence rate. Furthermore, proteomics analysis of PCR positive and negative naso-oropharyngeal samples provides information on the molecular level which highlights disease pathology. Samples from 15 PCR positive cases and 15 PCR negative cases were analyzed with nanoLC-MS/MS to identify the differentially expressed proteins. Proteomic analyses identified 207 proteins across the sample set and 17 of them were statistically significant. Protein-protein interaction analyses emphasized pathways like Neutrophil degranulation, Innate Immune System, Antimicrobial Peptides. Neutrophil Elastase (ELANE), Azurocidin (AZU1), Myeloperoxidase (MPO), Myeloblastin (PRTN3), Cathepsin G (CTSG) and Transcobalamine-1 (TCN1) were found to be significantly altered in naso-oropharyngeal samples of SARS-CoV-2 patients. The identified proteins are linked to alteration in the innate immune system specifically via neutrophil degranulation and NETosis.