tetano
Editor, Senior Moderator
[h=1]In vivo imaging of the pathophysiological changes and neutrophil dynamics in influenza virus-infected mouse lungs[/h] Hiroshi Ueki, I-Hsuan Wang, Satoshi Fukuyama, Hiroaki Katsura, Tiago Jose da Silva Lopes, Gabriele Neumann, and Yoshihiro Kawaoka
PNAS June 25, 2018. 201806265; published ahead of print June 25, 2018. https://doi.org/10.1073/pnas.1806265115
[h=2]Significance[/h] We used a state-of-the-art in vivo imaging system and fluorescent influenza viruses (Color-flu) to determine in real time the pathophysiological changes in the lungs of infected mice. We found that influenza virus infections reduced blood flow speed and decreased neutrophil motility. More significantly, infection with a prototypic ?bird flu? strain, a highly pathogenic H5N1 influenza virus, caused higher pulmonary permeability than did infection with a mouse-adapted human influenza virus. This in vivo imaging system with quantitative analyses allowed us to reveal the progression of the disease at the cellular level and to perform a multiparameter analysis that is not possible by using conventional histopathology.
[h=2]Abstract[/h] The pathophysiological changes that occur in lungs infected with influenza viruses are poorly understood. Here we established an in vivo imaging system that combines two-photon excitation microscopy and fluorescent influenza viruses of different pathogenicity. This approach allowed us to monitor and correlate several parameters and physiological changes including the spread of infection, pulmonary permeability, pulmonary perfusion speed, number of recruited neutrophils in infected lungs, and neutrophil motion in the lungs of live mice. Several physiological changes were larger and occurred earlier in mice infected with a highly pathogenic H5N1 influenza virus compared with those infected with a mouse-adapted human strain. These findings demonstrate the potential of our in vivo imaging system to provide novel information about the pathophysiological consequences of virus infections.
http://www.pnas.org/content/early/2018/06/21/1806265115
PNAS June 25, 2018. 201806265; published ahead of print June 25, 2018. https://doi.org/10.1073/pnas.1806265115
- Contributed by Yoshihiro Kawaoka, June 1, 2018 (sent for review April 23, 2018; reviewed by Daniel R. Perez and Stacey L. Schultz-Cherry
[h=2]Significance[/h] We used a state-of-the-art in vivo imaging system and fluorescent influenza viruses (Color-flu) to determine in real time the pathophysiological changes in the lungs of infected mice. We found that influenza virus infections reduced blood flow speed and decreased neutrophil motility. More significantly, infection with a prototypic ?bird flu? strain, a highly pathogenic H5N1 influenza virus, caused higher pulmonary permeability than did infection with a mouse-adapted human influenza virus. This in vivo imaging system with quantitative analyses allowed us to reveal the progression of the disease at the cellular level and to perform a multiparameter analysis that is not possible by using conventional histopathology.
[h=2]Abstract[/h] The pathophysiological changes that occur in lungs infected with influenza viruses are poorly understood. Here we established an in vivo imaging system that combines two-photon excitation microscopy and fluorescent influenza viruses of different pathogenicity. This approach allowed us to monitor and correlate several parameters and physiological changes including the spread of infection, pulmonary permeability, pulmonary perfusion speed, number of recruited neutrophils in infected lungs, and neutrophil motion in the lungs of live mice. Several physiological changes were larger and occurred earlier in mice infected with a highly pathogenic H5N1 influenza virus compared with those infected with a mouse-adapted human strain. These findings demonstrate the potential of our in vivo imaging system to provide novel information about the pathophysiological consequences of virus infections.
http://www.pnas.org/content/early/2018/06/21/1806265115