tetano
Editor, Senior Moderator
Vaccine. 2017 Apr 18. pii: S0264-410X(17)30469-3. doi: 10.1016/j.vaccine.2017.04.009. [Epub ahead of print]
[h=1]Potency of whole virus particle and split virion vaccines using dissolving microneedle against challenges of H1N1 and H5N1 influenza viruses in mice.[/h] Nakatsukasa A[SUP]1[/SUP], Kuruma K[SUP]2[/SUP], Okamatsu M[SUP]1[/SUP], Hiono T[SUP]1[/SUP], Suzuki M[SUP]1[/SUP], Matsuno K[SUP]3[/SUP], Kida H[SUP]4[/SUP], Oyamada T[SUP]2[/SUP], Sakoda Y[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Transdermal vaccination using a microneedle (MN) confers enhanced immunity compared with subcutaneous (SC) vaccination. Here we developed a novel dissolving MN patch for the influenza vaccine. The potencies of split virion and whole virus particle (WVP) vaccines prepared from A/Puerto Rico/8/1934 (H1N1) and A/duck/Hokkaido/Vac-3/2007 (H5N1), respectively, were evaluated. MN vaccination induced higher neutralizing antibody responses than SC vaccination in mice. Moreover, MN vaccination with a lower dose of antigens conferred protective immunity against lethal challenges of influenza viruses than SC vaccination in mice. These results suggest that the WVP vaccines administered using MN are an effective combination for influenza vaccine to be further validated in humans.
Copyright ? 2017 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Dissolving microneedle; Influenza; Split virion; Transdermal vaccination; Vaccine; Whole virus particle
PMID: 28431812 DOI: 10.1016/j.vaccine.2017.04.009
[h=1]Potency of whole virus particle and split virion vaccines using dissolving microneedle against challenges of H1N1 and H5N1 influenza viruses in mice.[/h] Nakatsukasa A[SUP]1[/SUP], Kuruma K[SUP]2[/SUP], Okamatsu M[SUP]1[/SUP], Hiono T[SUP]1[/SUP], Suzuki M[SUP]1[/SUP], Matsuno K[SUP]3[/SUP], Kida H[SUP]4[/SUP], Oyamada T[SUP]2[/SUP], Sakoda Y[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Transdermal vaccination using a microneedle (MN) confers enhanced immunity compared with subcutaneous (SC) vaccination. Here we developed a novel dissolving MN patch for the influenza vaccine. The potencies of split virion and whole virus particle (WVP) vaccines prepared from A/Puerto Rico/8/1934 (H1N1) and A/duck/Hokkaido/Vac-3/2007 (H5N1), respectively, were evaluated. MN vaccination induced higher neutralizing antibody responses than SC vaccination in mice. Moreover, MN vaccination with a lower dose of antigens conferred protective immunity against lethal challenges of influenza viruses than SC vaccination in mice. These results suggest that the WVP vaccines administered using MN are an effective combination for influenza vaccine to be further validated in humans.
Copyright ? 2017 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Dissolving microneedle; Influenza; Split virion; Transdermal vaccination; Vaccine; Whole virus particle
PMID: 28431812 DOI: 10.1016/j.vaccine.2017.04.009