tetano
Editor, Senior Moderator
PLoS One. 2012;7(12):e51029. doi: 10.1371/journal.pone.0051029. Epub 2012 Dec 10.
Potential Role of HPA Axis and Sympathetic Nervous Responses in Depletion of B Cells Induced by H9N2 Avian Influenza Virus Infection.
Qi W, Tian J, Zhang C, He J, Ning Z, Jiao P, Liao M.
Source
College of Veterinary Medicine, South China Agricultural University, Guangdong, People's Republic of China ; MOA Key Laboratory for Animal Vaccine Development, Guangzhou, People's Republic of China ; Key Lab of Zoonoses Control and Prevention of Guangdong, Guangdong, People's Republic of China.
Abstract
Except severe pulmonary disease caused by influenza virus infection, an impaired immune system is also a clinic characteristic. However, the mechanism(s) of influenza virus infection-induced depletion of B cells was unknown. Here, we compared the effect of two variant virulence H9N2 virus infections on mouse B cells. Our study found that the infection with highly pathogenic virus (V) of led to depletion of spleen B cells and bone marrow (BM) early B cells, compared to lowly pathogenic virus (Ts). Moreover, high apoptosis and cell cycle arrest in spleen and BM were detected, suggesting important factors for the reduction of B cells in both organs. Further, this effect was not caused by virus replication in spleen and BM. Compared to Ts virus infection, V virus resulted in higher glucocorticoids (GCs) and lower leptin level in plasma. Intraperitoneal GCs receptor antagonist RU486 injection was sufficient to prevent the loss of spleen B cell and BM pro- and immature B cells, but similar result was not observed in leptin-treated mice. Depletion of spleen B cells and BM pro-B cells was also reversed by chemical sympathectomy mediated by the norepinephrine (NE) analog 6-hydroxydopamine (6-OHDA), but the treatment didn't affect the GCs level. This study demonstrated that depletion of B cells induced by H9N2 AIV was dependent on HPA axis and sympathetic response.
PMID:
23251416
[PubMed - in process]
PMCID:
PMC3519482
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/23251416
Potential Role of HPA Axis and Sympathetic Nervous Responses in Depletion of B Cells Induced by H9N2 Avian Influenza Virus Infection.
Qi W, Tian J, Zhang C, He J, Ning Z, Jiao P, Liao M.
Source
College of Veterinary Medicine, South China Agricultural University, Guangdong, People's Republic of China ; MOA Key Laboratory for Animal Vaccine Development, Guangzhou, People's Republic of China ; Key Lab of Zoonoses Control and Prevention of Guangdong, Guangdong, People's Republic of China.
Abstract
Except severe pulmonary disease caused by influenza virus infection, an impaired immune system is also a clinic characteristic. However, the mechanism(s) of influenza virus infection-induced depletion of B cells was unknown. Here, we compared the effect of two variant virulence H9N2 virus infections on mouse B cells. Our study found that the infection with highly pathogenic virus (V) of led to depletion of spleen B cells and bone marrow (BM) early B cells, compared to lowly pathogenic virus (Ts). Moreover, high apoptosis and cell cycle arrest in spleen and BM were detected, suggesting important factors for the reduction of B cells in both organs. Further, this effect was not caused by virus replication in spleen and BM. Compared to Ts virus infection, V virus resulted in higher glucocorticoids (GCs) and lower leptin level in plasma. Intraperitoneal GCs receptor antagonist RU486 injection was sufficient to prevent the loss of spleen B cell and BM pro- and immature B cells, but similar result was not observed in leptin-treated mice. Depletion of spleen B cells and BM pro-B cells was also reversed by chemical sympathectomy mediated by the norepinephrine (NE) analog 6-hydroxydopamine (6-OHDA), but the treatment didn't affect the GCs level. This study demonstrated that depletion of B cells induced by H9N2 AIV was dependent on HPA axis and sympathetic response.
PMID:
23251416
[PubMed - in process]
PMCID:
PMC3519482
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/23251416