tetano
Editor, Senior Moderator
Clin Infect Dis. 2019 Jul 12. pii: ciz639. doi: 10.1093/cid/ciz639. [Epub ahead of print]
[h=1]Pre-existing anti-neuraminidase antibodies are associated with shortened duration of influenza A (H1N1)pdm virus shedding and illness in naturally infected adults.[/h] Maier HE[SUP]1[/SUP], Nachbagauer R[SUP]2[/SUP], Kuan G[SUP]3,[/SUP][SUP]4[/SUP], Ng S[SUP]1[/SUP], Lopez R[SUP]5,[/SUP][SUP]3[/SUP], Sanchez N[SUP]3[/SUP], Stadlbauer D[SUP]2[/SUP], Gresh L[SUP]3[/SUP], Schiller A[SUP]1[/SUP], Rajabhathor A[SUP]2[/SUP], Ojeda S[SUP]3[/SUP], Guglia AF[SUP]2[/SUP], Amanat F[SUP]2,[/SUP][SUP]6[/SUP], Balmaseda A[SUP]5,[/SUP][SUP]3[/SUP], Krammer F[SUP]2[/SUP], Gordon A[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza causes a substantial burden worldwide, and current seasonal influenza vaccine has suboptimal effectiveness. To develop better, more broadly protective vaccines, a more thorough understanding is needed of how antibodies that target the influenza virus surface antigens, hemagglutinin (HA; including head and stalk regions), and neuraminidase (NA), impact influenza illness and virus transmission.
[h=4]METHODS:[/h] We used a case-ascertained, community-based study of household influenza virus transmission set in Managua, Nicaragua. Using data from 170 RT-PCR-confirmed influenza virus A(H1N1)pdm infections and 45 household members with serologically-confirmed infection, we examined the association of pre-existing NA, hemagglutination inhibiting (HAI), and HA stalk antibody levels and influenza viral shedding and disease duration using accelerated failure time (AFT) models.
[h=4]RESULTS:[/h] Among RT-PCR-confirmed infections in adults, pre-existing anti-NA antibody levels of ≥40 were associated with a 69% (95%CI: 34%, 85%) shortened shedding duration (mean: 1.0 vs 3.2 days). NA antibody levels of ≥80 were associated with further shortened shedding and significantly shortened symptom duration (ILI: 82%, 95%CI: 39%, 95%). Among RT-PCR-confirmed infections in children, HAI titers of ≥1:20 were associated with a 32% (95%CI: 13%, 47%) shortened shedding duration (mean: 3.9 vs 6.0 days).
[h=4]CONCLUSIONS:[/h] Our results suggest that anti-NA antibodies play a large role in reducing influenza illness duration in adults and may impact transmission, most clearly among adults. Neuraminidase should be considered as an additional target in next-generation influenza virus vaccine development.
? The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America.
[h=4]KEYWORDS:[/h] hemagglutination inhibition (HAI); household; influenza; neuraminidase; shedding
PMID: 31300819 DOI: 10.1093/cid/ciz639
[h=1]Pre-existing anti-neuraminidase antibodies are associated with shortened duration of influenza A (H1N1)pdm virus shedding and illness in naturally infected adults.[/h] Maier HE[SUP]1[/SUP], Nachbagauer R[SUP]2[/SUP], Kuan G[SUP]3,[/SUP][SUP]4[/SUP], Ng S[SUP]1[/SUP], Lopez R[SUP]5,[/SUP][SUP]3[/SUP], Sanchez N[SUP]3[/SUP], Stadlbauer D[SUP]2[/SUP], Gresh L[SUP]3[/SUP], Schiller A[SUP]1[/SUP], Rajabhathor A[SUP]2[/SUP], Ojeda S[SUP]3[/SUP], Guglia AF[SUP]2[/SUP], Amanat F[SUP]2,[/SUP][SUP]6[/SUP], Balmaseda A[SUP]5,[/SUP][SUP]3[/SUP], Krammer F[SUP]2[/SUP], Gordon A[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza causes a substantial burden worldwide, and current seasonal influenza vaccine has suboptimal effectiveness. To develop better, more broadly protective vaccines, a more thorough understanding is needed of how antibodies that target the influenza virus surface antigens, hemagglutinin (HA; including head and stalk regions), and neuraminidase (NA), impact influenza illness and virus transmission.
[h=4]METHODS:[/h] We used a case-ascertained, community-based study of household influenza virus transmission set in Managua, Nicaragua. Using data from 170 RT-PCR-confirmed influenza virus A(H1N1)pdm infections and 45 household members with serologically-confirmed infection, we examined the association of pre-existing NA, hemagglutination inhibiting (HAI), and HA stalk antibody levels and influenza viral shedding and disease duration using accelerated failure time (AFT) models.
[h=4]RESULTS:[/h] Among RT-PCR-confirmed infections in adults, pre-existing anti-NA antibody levels of ≥40 were associated with a 69% (95%CI: 34%, 85%) shortened shedding duration (mean: 1.0 vs 3.2 days). NA antibody levels of ≥80 were associated with further shortened shedding and significantly shortened symptom duration (ILI: 82%, 95%CI: 39%, 95%). Among RT-PCR-confirmed infections in children, HAI titers of ≥1:20 were associated with a 32% (95%CI: 13%, 47%) shortened shedding duration (mean: 3.9 vs 6.0 days).
[h=4]CONCLUSIONS:[/h] Our results suggest that anti-NA antibodies play a large role in reducing influenza illness duration in adults and may impact transmission, most clearly among adults. Neuraminidase should be considered as an additional target in next-generation influenza virus vaccine development.
? The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America.
[h=4]KEYWORDS:[/h] hemagglutination inhibition (HAI); household; influenza; neuraminidase; shedding
PMID: 31300819 DOI: 10.1093/cid/ciz639