tetano
Editor, Senior Moderator
BMC Res Notes. 2016 Aug 30;9(1):423. doi: 10.1186/s13104-016-2235-8.
[h=1]Primer development to obtain complete coding sequence of HA and NA genes of influenza A/H3N2 virus.[/h] Agustiningsih A[SUP]1[/SUP], Trimarsanto H[SUP]2,[/SUP][SUP]3[/SUP], Setiawaty V[SUP]4[/SUP], Artika IM[SUP]2,[/SUP][SUP]5[/SUP], Muljono DH[SUP]2,[/SUP][SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza is an acute respiratory illness and has become a serious public health problem worldwide. The need to study the HA and NA genes in influenza A virus is essential since these genes frequently undergo mutations. This study describes the development of primer sets for RT-PCR to obtain complete coding sequence of Hemagglutinin (HA) and Neuraminidase (NA) genes of influenza A/H3N2 virus from Indonesia. The primers were developed based on influenza A/H3N2 sequence worldwide from Global Initiative on Sharing All Influenza Data (GISAID) and further tested using Indonesian influenza A/H3N2 archived samples of influenza-like illness (ILI) surveillance from 2008 to 2009.
[h=4]RESULTS:[/h] An optimum RT-PCR condition was acquired for all HA and NA fragments designed to cover complete coding sequence of HA and NA genes. A total of 71 samples were successfully sequenced for complete coding sequence both of HA and NA genes out of 145 samples of influenza A/H3N2 tested.
[h=4]CONCLUSIONS:[/h] The developed primer sets were suitable for obtaining complete coding sequences of HA and NA genes of Indonesian samples from 2008 to 2009.
[h=4]KEYWORDS:[/h] HA gene; Influenza A/H3N2 virus; NA gene; Primer set
PMID: 27576569 PMCID: PMC5004302 DOI: 10.1186/s13104-016-2235-8
[PubMed - in process] Free full text
[h=1]Primer development to obtain complete coding sequence of HA and NA genes of influenza A/H3N2 virus.[/h] Agustiningsih A[SUP]1[/SUP], Trimarsanto H[SUP]2,[/SUP][SUP]3[/SUP], Setiawaty V[SUP]4[/SUP], Artika IM[SUP]2,[/SUP][SUP]5[/SUP], Muljono DH[SUP]2,[/SUP][SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza is an acute respiratory illness and has become a serious public health problem worldwide. The need to study the HA and NA genes in influenza A virus is essential since these genes frequently undergo mutations. This study describes the development of primer sets for RT-PCR to obtain complete coding sequence of Hemagglutinin (HA) and Neuraminidase (NA) genes of influenza A/H3N2 virus from Indonesia. The primers were developed based on influenza A/H3N2 sequence worldwide from Global Initiative on Sharing All Influenza Data (GISAID) and further tested using Indonesian influenza A/H3N2 archived samples of influenza-like illness (ILI) surveillance from 2008 to 2009.
[h=4]RESULTS:[/h] An optimum RT-PCR condition was acquired for all HA and NA fragments designed to cover complete coding sequence of HA and NA genes. A total of 71 samples were successfully sequenced for complete coding sequence both of HA and NA genes out of 145 samples of influenza A/H3N2 tested.
[h=4]CONCLUSIONS:[/h] The developed primer sets were suitable for obtaining complete coding sequences of HA and NA genes of Indonesian samples from 2008 to 2009.
[h=4]KEYWORDS:[/h] HA gene; Influenza A/H3N2 virus; NA gene; Primer set
PMID: 27576569 PMCID: PMC5004302 DOI: 10.1186/s13104-016-2235-8
[PubMed - in process] Free full text