tetano
Editor, Senior Moderator
Proc Natl Acad Sci U S A
. 2020 Oct 6;202014352.
doi: 10.1073/pnas.2014352117. Online ahead of print.
Decoy nanoparticles protect against COVID-19 by concurrently adsorbing viruses and inflammatory cytokines
Lang Rao[SUP] 1 [/SUP], Shuai Xia[SUP] 2 [/SUP], Wei Xu[SUP] 2 [/SUP], Rui Tian[SUP] 1 [/SUP], Guocan Yu[SUP] 1 [/SUP], Chenjian Gu[SUP] 2 [/SUP], Pan Pan[SUP] 3 4 [/SUP], Qian-Fang Meng[SUP] 5 [/SUP], Xia Cai[SUP] 2 [/SUP], Di Qu[SUP] 2 [/SUP], Lu Lu[SUP] 6 [/SUP], Youhua Xie[SUP] 6 [/SUP], Shibo Jiang[SUP] 6 [/SUP], Xiaoyuan Chen[SUP] 7 [/SUP]
Affiliations
Abstract
The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has highlighted the urgent need to rapidly develop therapeutic strategies for such emerging viruses without effective vaccines or drugs. Here, we report a decoy nanoparticle against COVID-19 through a powerful two-step neutralization approach: virus neutralization in the first step followed by cytokine neutralization in the second step. The nanodecoy, made by fusing cellular membrane nanovesicles derived from human monocytes and genetically engineered cells stably expressing angiotensin converting enzyme II (ACE2) receptors, possesses an antigenic exterior the same as source cells. By competing with host cells for virus binding, these nanodecoys effectively protect host cells from the infection of pseudoviruses and authentic SARS-CoV-2. Moreover, relying on abundant cytokine receptors on the surface, the nanodecoys efficiently bind and neutralize inflammatory cytokines including interleukin 6 (IL-6) and granulocyte-macrophage colony-stimulating factor (GM-CSF), and significantly suppress immune disorder and lung injury in an acute pneumonia mouse model. Our work presents a simple, safe, and robust antiviral nanotechnology for ongoing COVID-19 and future potential epidemics.
Keywords: COVID-19; SARS-CoV-2; cell membrane vesicle; cytokine storm; nanodecoy.
. 2020 Oct 6;202014352.
doi: 10.1073/pnas.2014352117. Online ahead of print.
Decoy nanoparticles protect against COVID-19 by concurrently adsorbing viruses and inflammatory cytokines
Lang Rao[SUP] 1 [/SUP], Shuai Xia[SUP] 2 [/SUP], Wei Xu[SUP] 2 [/SUP], Rui Tian[SUP] 1 [/SUP], Guocan Yu[SUP] 1 [/SUP], Chenjian Gu[SUP] 2 [/SUP], Pan Pan[SUP] 3 4 [/SUP], Qian-Fang Meng[SUP] 5 [/SUP], Xia Cai[SUP] 2 [/SUP], Di Qu[SUP] 2 [/SUP], Lu Lu[SUP] 6 [/SUP], Youhua Xie[SUP] 6 [/SUP], Shibo Jiang[SUP] 6 [/SUP], Xiaoyuan Chen[SUP] 7 [/SUP]
Affiliations
- PMID: 33024017
- DOI: 10.1073/pnas.2014352117
Abstract
The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has highlighted the urgent need to rapidly develop therapeutic strategies for such emerging viruses without effective vaccines or drugs. Here, we report a decoy nanoparticle against COVID-19 through a powerful two-step neutralization approach: virus neutralization in the first step followed by cytokine neutralization in the second step. The nanodecoy, made by fusing cellular membrane nanovesicles derived from human monocytes and genetically engineered cells stably expressing angiotensin converting enzyme II (ACE2) receptors, possesses an antigenic exterior the same as source cells. By competing with host cells for virus binding, these nanodecoys effectively protect host cells from the infection of pseudoviruses and authentic SARS-CoV-2. Moreover, relying on abundant cytokine receptors on the surface, the nanodecoys efficiently bind and neutralize inflammatory cytokines including interleukin 6 (IL-6) and granulocyte-macrophage colony-stimulating factor (GM-CSF), and significantly suppress immune disorder and lung injury in an acute pneumonia mouse model. Our work presents a simple, safe, and robust antiviral nanotechnology for ongoing COVID-19 and future potential epidemics.
Keywords: COVID-19; SARS-CoV-2; cell membrane vesicle; cytokine storm; nanodecoy.