tetano
Editor, Senior Moderator
Front Bioeng Biotechnol. 2015 Dec 8;3:197. doi: 10.3389/fbioe.2015.00197. eCollection 2015.
[h=1]Production of H5N1 Influenza Virus Matrix Protein 2 Ectodomain Protein Bodies in Tobacco Plants and in Insect Cells as a Candidate Universal Influenza Vaccine.[/h] Mbewana S[SUP]1[/SUP], Mortimer E[SUP]1[/SUP], P?ra FF[SUP]1[/SUP], Hitzeroth II[SUP]1[/SUP], Rybicki EP[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The spread of influenza A viruses is partially controlled and prevented by vaccination. The matrix protein 2 ectodomain (M2e) is the most conserved sequence in influenza A viruses, and is therefore a good potential target for a vaccine to protect against multiple virus subtypes. We explored the feasibility of an M2e-based universal influenza A vaccine candidate based on the highly pathogenic avian influenza A virus, H5N1. A synthetic M2e gene was human- and plant-codon optimized and fused in-frame with a sequence encoding the N-terminal proline-rich domain (Zera(?)) of the γ-zein protein of maize. Zera(?)M2e was expressed transiently in Nicotiana benthamiana and Sf21 baculovirus/insect cell expression systems, and Zera(?)M2e protein bodies (PBs) were successfully produced in both expression systems. The plant-produced Zera(?)M2e PBs were purified and injected into Balb/c mice. Western blot analysis using insect cell-produced Zera(?)M2e PBs and multiple tandem M2e sequences (5xM2e) fused with the avian influenza H5N1 transmembrane and cytosolic tail (5xM2e_tHA) confirmed the presence of M2e-specific antibodies in immunized mice sera. The immunogenicity of the Zera(?)M2e indicates that our plant-produced protein has potential as an inexpensive universal influenza A vaccine.
[h=4]KEYWORDS:[/h] M2e; influenza A virus; insect cell expression; plant expression; vaccine
PMID: 26697423 [PubMed] PMCID: PMC4672040 Free full text
[h=1]Production of H5N1 Influenza Virus Matrix Protein 2 Ectodomain Protein Bodies in Tobacco Plants and in Insect Cells as a Candidate Universal Influenza Vaccine.[/h] Mbewana S[SUP]1[/SUP], Mortimer E[SUP]1[/SUP], P?ra FF[SUP]1[/SUP], Hitzeroth II[SUP]1[/SUP], Rybicki EP[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The spread of influenza A viruses is partially controlled and prevented by vaccination. The matrix protein 2 ectodomain (M2e) is the most conserved sequence in influenza A viruses, and is therefore a good potential target for a vaccine to protect against multiple virus subtypes. We explored the feasibility of an M2e-based universal influenza A vaccine candidate based on the highly pathogenic avian influenza A virus, H5N1. A synthetic M2e gene was human- and plant-codon optimized and fused in-frame with a sequence encoding the N-terminal proline-rich domain (Zera(?)) of the γ-zein protein of maize. Zera(?)M2e was expressed transiently in Nicotiana benthamiana and Sf21 baculovirus/insect cell expression systems, and Zera(?)M2e protein bodies (PBs) were successfully produced in both expression systems. The plant-produced Zera(?)M2e PBs were purified and injected into Balb/c mice. Western blot analysis using insect cell-produced Zera(?)M2e PBs and multiple tandem M2e sequences (5xM2e) fused with the avian influenza H5N1 transmembrane and cytosolic tail (5xM2e_tHA) confirmed the presence of M2e-specific antibodies in immunized mice sera. The immunogenicity of the Zera(?)M2e indicates that our plant-produced protein has potential as an inexpensive universal influenza A vaccine.
[h=4]KEYWORDS:[/h] M2e; influenza A virus; insect cell expression; plant expression; vaccine
PMID: 26697423 [PubMed] PMCID: PMC4672040 Free full text