tetano
Editor, Senior Moderator
J Gen Virol. 2016 Oct 28. doi: 10.1099/jgv.0.000648. [Epub ahead of print]
[h=1]Progressive glycosylation of the hemagglutinin of avian influenza H5N1 modulates virus replication, virulence and chicken-to-chicken transmission without significant impact on antigenic drift.[/h] Abdelwhab EM[SUP]1[/SUP], Veits J[SUP]2[/SUP], Tauscher K[SUP]3[/SUP], Ziller M[SUP]4[/SUP], Grund C[SUP]5[/SUP], Hassan MK[SUP]6[/SUP], Shaheen M[SUP]7[/SUP], Harder TC[SUP]8[/SUP], Teifke JP[SUP]9[/SUP], Stech J[SUP]10[/SUP], Mettenleiter TC[SUP]11[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Highly pathogenic H5N1 avian influenza virus (A/H5N1) devastated the poultry industry and continues to pose a pandemic threat. Studying the progressive genetic changes in A/H5N1 after long term circulation in poultry may help to better understand A/H5N1 biology in birds. A/H5N1 clade 2.2.1.1 antigenic drift viruses have been isolated from vaccinated commercial poultry in Egypt. They exhibit a peculiar stepwise accumulation of glycosylation sites (GS) in the hemagglutinin (HA) with viruses carrying, beyond the conserved 5 GS, additional GS at amino acid residues 72, 154, 236 and 273 resulting in 6, 7, 8 or 9 GS in the HA. Available information about the impact of glycosylation on virus fitness and pathobiology is mostly derived from mammalian models. Here, we generated recombinant viruses imitating the progressive acquisition of GS in HA and investigated their biological relevance in vitro and in vivo. Our in vitro results indicated that the accumulation of GS correlated with increased glycosylation, increased virus replication, neuraminidase activity, cell-to-cell spread and thermostability, however, strikingly, without significant impact on virus escape from neutralizing antibodies. In vivo, glycosylation modulated virus virulence, tissue tropism, replication and chicken-to-chicken transmission. Predominance in the field was toward viruses with hyperglycosylated HA. Together, progressive glycosylation of the HA may foster persistence of A/H5N1 by increasing replication, stability and bird-to-bird transmission without significant impact on antigenic drift.
PMID: 27902339 DOI: 10.1099/jgv.0.000648
[PubMed - as supplied by publisher]
[h=1]Progressive glycosylation of the hemagglutinin of avian influenza H5N1 modulates virus replication, virulence and chicken-to-chicken transmission without significant impact on antigenic drift.[/h] Abdelwhab EM[SUP]1[/SUP], Veits J[SUP]2[/SUP], Tauscher K[SUP]3[/SUP], Ziller M[SUP]4[/SUP], Grund C[SUP]5[/SUP], Hassan MK[SUP]6[/SUP], Shaheen M[SUP]7[/SUP], Harder TC[SUP]8[/SUP], Teifke JP[SUP]9[/SUP], Stech J[SUP]10[/SUP], Mettenleiter TC[SUP]11[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Highly pathogenic H5N1 avian influenza virus (A/H5N1) devastated the poultry industry and continues to pose a pandemic threat. Studying the progressive genetic changes in A/H5N1 after long term circulation in poultry may help to better understand A/H5N1 biology in birds. A/H5N1 clade 2.2.1.1 antigenic drift viruses have been isolated from vaccinated commercial poultry in Egypt. They exhibit a peculiar stepwise accumulation of glycosylation sites (GS) in the hemagglutinin (HA) with viruses carrying, beyond the conserved 5 GS, additional GS at amino acid residues 72, 154, 236 and 273 resulting in 6, 7, 8 or 9 GS in the HA. Available information about the impact of glycosylation on virus fitness and pathobiology is mostly derived from mammalian models. Here, we generated recombinant viruses imitating the progressive acquisition of GS in HA and investigated their biological relevance in vitro and in vivo. Our in vitro results indicated that the accumulation of GS correlated with increased glycosylation, increased virus replication, neuraminidase activity, cell-to-cell spread and thermostability, however, strikingly, without significant impact on virus escape from neutralizing antibodies. In vivo, glycosylation modulated virus virulence, tissue tropism, replication and chicken-to-chicken transmission. Predominance in the field was toward viruses with hyperglycosylated HA. Together, progressive glycosylation of the HA may foster persistence of A/H5N1 by increasing replication, stability and bird-to-bird transmission without significant impact on antigenic drift.
PMID: 27902339 DOI: 10.1099/jgv.0.000648
[PubMed - as supplied by publisher]