tetano
Editor, Senior Moderator
J Vet Sci. 2014 Mar 21. [Epub ahead of print]
Protective efficacy of a high-growth reassortant swine H3N2 inactivated vaccine constructed by reverse genetic manipulation.
Wen F, Ma JH, Yu H, Yang FR, Huang M, Zhou YJ, Li ZJ, Tong GZ.
Abstract
Novel reassortant H3N2 swine influenza viruses (SwIV) with the matrix (M) gene from the 2009 H1N1 pandemic virus were isolated in many countries and outbreaks in multiple U.S. States, indicating the H3N2 subtype SwIV-infected pigs are posing a potential threat to public health. Considering that southern China is the world's largest producer of pigs, efficient vaccines should be developed to prevent pigs from acquiring H3N2 subtype SwIV infections, and thus to limit the possibility of SwIV infection at agricultural fairs. In this study, a high-growth reassortant virus (GD/PR8) was generated by plasmid-based reverse genetics, and tested as a candidate seed virus for the preparation of an inactivated vaccine. The protective efficacy of this vaccine was evaluated in mice by challenged them with another H3N2 SwIV isolate [A/Swine/Heilongjiang/1/05 (H3N2) (HLJ/05)]. Prime and boost inoculation of GD/PR8 vaccine yielded high-titer serum hemagglutination inhibiting (HI) antibodies and IgG antibodies. Complete protection of mice against H3N2 SwIV was observed, with significantly reduced lung lesion and viral loads in vaccine-inoculated mice compared with Mock-vaccinated controls. These results suggest that the GD/PR8 vaccine may serve as a promising vaccine candidate for rapid intervention of H3N2 SwIV outbreaks in China.
PMID:
24675833
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24675833
Protective efficacy of a high-growth reassortant swine H3N2 inactivated vaccine constructed by reverse genetic manipulation.
Wen F, Ma JH, Yu H, Yang FR, Huang M, Zhou YJ, Li ZJ, Tong GZ.
Abstract
Novel reassortant H3N2 swine influenza viruses (SwIV) with the matrix (M) gene from the 2009 H1N1 pandemic virus were isolated in many countries and outbreaks in multiple U.S. States, indicating the H3N2 subtype SwIV-infected pigs are posing a potential threat to public health. Considering that southern China is the world's largest producer of pigs, efficient vaccines should be developed to prevent pigs from acquiring H3N2 subtype SwIV infections, and thus to limit the possibility of SwIV infection at agricultural fairs. In this study, a high-growth reassortant virus (GD/PR8) was generated by plasmid-based reverse genetics, and tested as a candidate seed virus for the preparation of an inactivated vaccine. The protective efficacy of this vaccine was evaluated in mice by challenged them with another H3N2 SwIV isolate [A/Swine/Heilongjiang/1/05 (H3N2) (HLJ/05)]. Prime and boost inoculation of GD/PR8 vaccine yielded high-titer serum hemagglutination inhibiting (HI) antibodies and IgG antibodies. Complete protection of mice against H3N2 SwIV was observed, with significantly reduced lung lesion and viral loads in vaccine-inoculated mice compared with Mock-vaccinated controls. These results suggest that the GD/PR8 vaccine may serve as a promising vaccine candidate for rapid intervention of H3N2 SwIV outbreaks in China.
PMID:
24675833
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24675833