tetano
Editor, Senior Moderator
Clin Vaccine Immunol. 2015 Apr 1. pii: CVI.00091-15. [Epub ahead of print]
[h=1]Protective efficacy of the conserved NP, PB1, and M1 proteins as immunogens in DNA- and vaccinia virus-based universal influenza A virus vaccines in mice.[/h] Wang W[SUP]1[/SUP], Li R[SUP]2[/SUP], Deng Y[SUP]1[/SUP], Lu N[SUP]1[/SUP], Chen H[SUP]1[/SUP], Meng X[SUP]1[/SUP], Wang W[SUP]1[/SUP], Wang X[SUP]1[/SUP], Yan K[SUP]1[/SUP], Qi X[SUP]1[/SUP], Zhang X[SUP]1[/SUP], Xin W[SUP]1[/SUP], Lu Z[SUP]1[/SUP], Li X[SUP]1[/SUP], Bian T[SUP]1[/SUP], Gao Y[SUP]1[/SUP], Tan W[SUP]1[/SUP], Ruan L[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The conventional HA and NA-based influenza vaccines need to be updated most years, and are ineffective if the glycoprotein HA of the vaccine strains is a mismatch to that of the epidemic strain. Universal vaccines targeting conserved viral components might provide cross protection, and thus complement and improve conventional vaccines. In this study, we generated DNA plasmids and recombinant vaccinia viruses expressing the conserved proteins NP, PB1, and M1 from influenza virus A/Beijing/30/95 (H3N2). BALB/c mice were immunized intramuscularly with a single vaccine based on NP, PB1, or M1 alone or a combination vaccine based on all three antigens, were then challenged with lethal doses of the heterologous influenza virus A/PR/8/34 (H1N1). Vaccines based on NP, PB1, and M1 provided complete or partial protection against 1.7 LD50 PR8 challenge in mice. Among the three antigens, NP-based vaccines induced protection against 5 LD50 and 10 LD50, and thus exhibited the greatest protective effect. Universal influenza vaccines based on the combination of NP, PB1, and M1 induced a strong immune response, and thus might be an alternative approach to addressing future influenza virus pandemics.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 25834017 [PubMed - as supplied by publisher]
[h=1]Protective efficacy of the conserved NP, PB1, and M1 proteins as immunogens in DNA- and vaccinia virus-based universal influenza A virus vaccines in mice.[/h] Wang W[SUP]1[/SUP], Li R[SUP]2[/SUP], Deng Y[SUP]1[/SUP], Lu N[SUP]1[/SUP], Chen H[SUP]1[/SUP], Meng X[SUP]1[/SUP], Wang W[SUP]1[/SUP], Wang X[SUP]1[/SUP], Yan K[SUP]1[/SUP], Qi X[SUP]1[/SUP], Zhang X[SUP]1[/SUP], Xin W[SUP]1[/SUP], Lu Z[SUP]1[/SUP], Li X[SUP]1[/SUP], Bian T[SUP]1[/SUP], Gao Y[SUP]1[/SUP], Tan W[SUP]1[/SUP], Ruan L[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The conventional HA and NA-based influenza vaccines need to be updated most years, and are ineffective if the glycoprotein HA of the vaccine strains is a mismatch to that of the epidemic strain. Universal vaccines targeting conserved viral components might provide cross protection, and thus complement and improve conventional vaccines. In this study, we generated DNA plasmids and recombinant vaccinia viruses expressing the conserved proteins NP, PB1, and M1 from influenza virus A/Beijing/30/95 (H3N2). BALB/c mice were immunized intramuscularly with a single vaccine based on NP, PB1, or M1 alone or a combination vaccine based on all three antigens, were then challenged with lethal doses of the heterologous influenza virus A/PR/8/34 (H1N1). Vaccines based on NP, PB1, and M1 provided complete or partial protection against 1.7 LD50 PR8 challenge in mice. Among the three antigens, NP-based vaccines induced protection against 5 LD50 and 10 LD50, and thus exhibited the greatest protective effect. Universal influenza vaccines based on the combination of NP, PB1, and M1 induced a strong immune response, and thus might be an alternative approach to addressing future influenza virus pandemics.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 25834017 [PubMed - as supplied by publisher]