tetano
Editor, Senior Moderator
Analyst. 2013 Jul 3. [Epub ahead of print]
Protein-binding aptamer assisted signal amplification for the detection of influenza A (H1N1) DNA sequences based on quantum dot fluorescence polarization analysis.
Zhang J, Tian J, He Y, Chen S, Jiang Y, Zhao Y, Zhao S.
Source
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin, 541004, China. birdtjn@sina.com.
Abstract
We report a fluorescence polarization platform for H1N1 detection based on the construction of a DNA functional QD fluorescence polarization probe and a bi-functional protein binding aptamer (Apt-DNA). The assay has a linear range from 10 nM to 100 nM with a detection limit of 3.45 nM and is selective over the mismatched bases.
PMID:
23826611
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23826611
Protein-binding aptamer assisted signal amplification for the detection of influenza A (H1N1) DNA sequences based on quantum dot fluorescence polarization analysis.
Zhang J, Tian J, He Y, Chen S, Jiang Y, Zhao Y, Zhao S.
Source
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering of Guangxi Normal University, Guilin, 541004, China. birdtjn@sina.com.
Abstract
We report a fluorescence polarization platform for H1N1 detection based on the construction of a DNA functional QD fluorescence polarization probe and a bi-functional protein binding aptamer (Apt-DNA). The assay has a linear range from 10 nM to 100 nM with a detection limit of 3.45 nM and is selective over the mismatched bases.
PMID:
23826611
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23826611