tetano
Editor, Senior Moderator
Protein Sci
. 2020 Jul 25.
doi: 10.1002/pro.3918. Online ahead of print.
Drivers of recombinant soluble influenza A virus hemagglutinin and neuraminidase expression in mammalian cells
Roosmarijn van der Woude[SUP] 1 [/SUP], Hannah L Turner[SUP] 2 [/SUP], Ilhan Tomris[SUP] 1 [/SUP], Kim M Bouwman[SUP] 1 [/SUP], Andrew B Ward[SUP] 2 [/SUP], Robert P de Vries[SUP] 1 [/SUP]
Affiliations
Abstract
Recombinant soluble trimeric influenza A virus hemagglutinins (HA) and tetrameric neuraminidases (NA) have proven to be excellent tools to decipher biological properties. Receptor binding and sialic acid cleavage by recombinant proteins correlate satisfactorily compared to whole viruses. Expression of HA and NA can be achieved in a plethora of different laboratory hosts. For immunological and receptor interaction studies however, insect and mammalian cell expressed proteins are preferred due to the presence of N-linked glycosylation and disulfide bond formation. Because mammalian-cell expression is widely applied, an increased expression yield is an important goal. Here we report that using codon-optimized genes and sfGFP fusions, the expression yield of HA can be significantly improved. sfGFP also significantly increased expression yields when fused to the N-terminus of NA. In this study, a suite of different hemagglutinin and neuraminidase constructs are described, which can be valuable tools to study a wide array of different HAs, NAs and their mutants. This article is protected by copyright. All rights reserved.
Keywords: Influenza A Virus; hemagglutinin; multimerization domain; neuraminidase; super folder green fluorescent protein.
. 2020 Jul 25.
doi: 10.1002/pro.3918. Online ahead of print.
Drivers of recombinant soluble influenza A virus hemagglutinin and neuraminidase expression in mammalian cells
Roosmarijn van der Woude[SUP] 1 [/SUP], Hannah L Turner[SUP] 2 [/SUP], Ilhan Tomris[SUP] 1 [/SUP], Kim M Bouwman[SUP] 1 [/SUP], Andrew B Ward[SUP] 2 [/SUP], Robert P de Vries[SUP] 1 [/SUP]
Affiliations
- PMID: 32710576
- DOI: 10.1002/pro.3918
Abstract
Recombinant soluble trimeric influenza A virus hemagglutinins (HA) and tetrameric neuraminidases (NA) have proven to be excellent tools to decipher biological properties. Receptor binding and sialic acid cleavage by recombinant proteins correlate satisfactorily compared to whole viruses. Expression of HA and NA can be achieved in a plethora of different laboratory hosts. For immunological and receptor interaction studies however, insect and mammalian cell expressed proteins are preferred due to the presence of N-linked glycosylation and disulfide bond formation. Because mammalian-cell expression is widely applied, an increased expression yield is an important goal. Here we report that using codon-optimized genes and sfGFP fusions, the expression yield of HA can be significantly improved. sfGFP also significantly increased expression yields when fused to the N-terminus of NA. In this study, a suite of different hemagglutinin and neuraminidase constructs are described, which can be valuable tools to study a wide array of different HAs, NAs and their mutants. This article is protected by copyright. All rights reserved.
Keywords: Influenza A Virus; hemagglutinin; multimerization domain; neuraminidase; super folder green fluorescent protein.