tetano
Editor, Senior Moderator
Virus Res. 2013 Feb 19. pii: S0168-1702(13)00050-6. doi: 10.1016/j.virusres.2013.02.001. [Epub ahead of print]
Proteins of duck influenza virus responsible for acquisition of pathogenicity in chickens.
Yamamoto N, Soda K, Sakoda Y, Okamatsu M, Kida H.
Source
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan.
Abstract
Influenza virus rgVac1sub-P0 (H5N1) (rgVac1-P0), in which a pair of dibasic amino acid residues was introduced at the cleavage site of the HA of a reassortant of H5N2 and H7N1 viruses of duck origin, was low pathogenic in chickens. Vac1sub-P3 (H5N1) (Vac1-P3) was selected as a highly pathogenic avian influenza virus by 3 consecutive passages in chickens from low pathogenic strain rgVac1-P0. Comparison of amino acid sequences of the virus proteins and experimental infection of chickens with a series of recombinant viruses demonstrated that in addition to the HA, each of the PA, NP, M1, and M2 of Vac1-P3 are responsible for the acquisition of pathogenicity in chickens. These 4 proteins of Vac1-P3 synergistically contributed to efficient virus replication in chickens.
Copyright ? 2013 Elsevier B.V. All rights reserved.
PMID:
23434880
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23434880
Proteins of duck influenza virus responsible for acquisition of pathogenicity in chickens.
Yamamoto N, Soda K, Sakoda Y, Okamatsu M, Kida H.
Source
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan.
Abstract
Influenza virus rgVac1sub-P0 (H5N1) (rgVac1-P0), in which a pair of dibasic amino acid residues was introduced at the cleavage site of the HA of a reassortant of H5N2 and H7N1 viruses of duck origin, was low pathogenic in chickens. Vac1sub-P3 (H5N1) (Vac1-P3) was selected as a highly pathogenic avian influenza virus by 3 consecutive passages in chickens from low pathogenic strain rgVac1-P0. Comparison of amino acid sequences of the virus proteins and experimental infection of chickens with a series of recombinant viruses demonstrated that in addition to the HA, each of the PA, NP, M1, and M2 of Vac1-P3 are responsible for the acquisition of pathogenicity in chickens. These 4 proteins of Vac1-P3 synergistically contributed to efficient virus replication in chickens.
Copyright ? 2013 Elsevier B.V. All rights reserved.
PMID:
23434880
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23434880