tetano
Editor, Senior Moderator
Vaccine. 2017 Sep 6. pii: S0264-410X(17)31151-9. doi: 10.1016/j.vaccine.2017.08.053. [Epub ahead of print]
[h=1]Proteomic analysis of influenza haemagglutinin-specific antibodies following vaccination reveals convergent immunoglobulin variable region signatures.[/h] Adamson PJ[SUP]1[/SUP], Al Kindi MA[SUP]2[/SUP], Wang JJ[SUP]3[/SUP], Colella AD[SUP]4[/SUP], Chataway TK[SUP]5[/SUP], Petrovsky N[SUP]6[/SUP], Gordon TP[SUP]7[/SUP], Gordon DL[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Analysis of the anti-haemagglutinin serum antibody proteome from six H1N1pdm09 influenza A vaccinated subjects demonstrated restricted IgG1 heavy chain species encoded by IGHV5-51 and IGHV3-7 gene families in 2 subjects and either IGHV5-51 or IGHV3-7 in 4 individuals. All subjects exhibited a dominant IGKV3-20 light chain, however 5 subjects also exhibited IGKV3-11 and IGKV4-1 families. Sequences were closely aligned with the matched germline sequence, with few shared mutations. This study illustrates the feasibility of using a proteomic approach to determine the expressed V region signatures of serum antibodies induced by vaccination.
Copyright ? 2017 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza A; Mass spectrometric sequencing; Proteomics; Serum antibody repertoire; Vaccination
PMID: 28888340 DOI: 10.1016/j.vaccine.2017.08.053
[h=1]Proteomic analysis of influenza haemagglutinin-specific antibodies following vaccination reveals convergent immunoglobulin variable region signatures.[/h] Adamson PJ[SUP]1[/SUP], Al Kindi MA[SUP]2[/SUP], Wang JJ[SUP]3[/SUP], Colella AD[SUP]4[/SUP], Chataway TK[SUP]5[/SUP], Petrovsky N[SUP]6[/SUP], Gordon TP[SUP]7[/SUP], Gordon DL[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Analysis of the anti-haemagglutinin serum antibody proteome from six H1N1pdm09 influenza A vaccinated subjects demonstrated restricted IgG1 heavy chain species encoded by IGHV5-51 and IGHV3-7 gene families in 2 subjects and either IGHV5-51 or IGHV3-7 in 4 individuals. All subjects exhibited a dominant IGKV3-20 light chain, however 5 subjects also exhibited IGKV3-11 and IGKV4-1 families. Sequences were closely aligned with the matched germline sequence, with few shared mutations. This study illustrates the feasibility of using a proteomic approach to determine the expressed V region signatures of serum antibodies induced by vaccination.
Copyright ? 2017 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza A; Mass spectrometric sequencing; Proteomics; Serum antibody repertoire; Vaccination
PMID: 28888340 DOI: 10.1016/j.vaccine.2017.08.053