tetano
Editor, Senior Moderator
Microbiol Immunol. 2012 Apr 24. doi: 10.1111/j.1348-0421.2012.00468.x. [Epub ahead of print]
Purification of human and avian influenza viruses using cellulose sulfate ester (Cellufine(?) Sulfate) for application in the process of vaccine production.
Sakoda Y, Okamatsu M, Isoda N, Yamamoto N, Ozaki K, Umeda Y, Aoyama S, Kida H.
Source
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan Technical Support Team, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan Minamata Headquarters, JNC Corporation, 867-8501, Minamata, Kumamoto, Japan Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.
Abstract
Affinity chromatography using sulfated, spherical cellulose beads (Cellufine(?) Sulfate) was assessed for the purification of influenza A and influenza B viruses. Recovery rates of viruses eluted from the beads were high for all tested virus strains. This method was also useful to remove chicken egg-derived impurities from allantoic fluids containing influenza viruses, the hemagglutination activity per amount of protein in the eluted sample was significantly higher than that in the applied sample. These results suggest that the purification of influenza viruses using Cellufine(?) Sulfate is a practical method as a primary purification in the process of influenza vaccine production.
? 2012 The Societies and Blackwell Publishing Asia Pty Ltd.
PMID:
22530951
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22530951
Purification of human and avian influenza viruses using cellulose sulfate ester (Cellufine(?) Sulfate) for application in the process of vaccine production.
Sakoda Y, Okamatsu M, Isoda N, Yamamoto N, Ozaki K, Umeda Y, Aoyama S, Kida H.
Source
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan Technical Support Team, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan Minamata Headquarters, JNC Corporation, 867-8501, Minamata, Kumamoto, Japan Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.
Abstract
Affinity chromatography using sulfated, spherical cellulose beads (Cellufine(?) Sulfate) was assessed for the purification of influenza A and influenza B viruses. Recovery rates of viruses eluted from the beads were high for all tested virus strains. This method was also useful to remove chicken egg-derived impurities from allantoic fluids containing influenza viruses, the hemagglutination activity per amount of protein in the eluted sample was significantly higher than that in the applied sample. These results suggest that the purification of influenza viruses using Cellufine(?) Sulfate is a practical method as a primary purification in the process of influenza vaccine production.
? 2012 The Societies and Blackwell Publishing Asia Pty Ltd.
PMID:
22530951
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22530951