tetano
Editor, Senior Moderator
Vaccine. 2014 Mar 11. pii: S0264-410X(14)00313-2. doi: 10.1016/j.vaccine.2014.02.086. [Epub ahead of print]
Purification of Vero cell derived live replication deficient influenza A and B virus by ion exchange monolith chromatography.
Banjac M1, Roethl E2, Gelhart F2, Kramberger P1, Jarc BL1, Jarc M1, Strancar A1, Muster T2, Peterka M3.
Author information
Abstract
We explored the possibilities for purification of various ΔNS1 live, replication deficient influenza viruses on ion exchange methacrylate monoliths. Influenza A ΔNS1-H1N1, ΔNS1-H3N2, ΔNS1-H5N1 and ΔNS1-influenza B viruses were propagated in Vero cells and concentrated by tangential flow filtration. All four virus strains adsorbed well to CIM QA and CIM DEAE anion exchangers, with CIM QA producing higher recoveries than CIM DEAE. ΔNS1-influenza A viruses adsorbed well also to CIM SO3 cation exchanger at the same pH, while ΔNS1-influenza B virus adsorption to CIM SO3 was not complete. Dynamic binding capacity (DBC) for CIM QA, DEAE and SO3 methacrylate monoliths for influenza A ΔNS1-H1N1 virus were 2.00E+10 TCID50/ml, 1.12E+10 TCID50/ml and 9.00E+08 TCID50/ml, respectively. Purification of ΔNS1 viruses on CIM QA was scaled up and reproducibility was confirmed. Yields of infectious virus on CIM QA were between 70.8?32.3% and 87?30.8%. Total protein removal varied from 93.3?0.4% to 98.6?0.2% and host cell DNA removal efficiency was ranging from 76.4% to 99.9% and strongly depended on pretreatment with deoxyribonuclease.
Copyright ? 2014. Published by Elsevier Ltd.
KEYWORDS:
Chromatography, Influenza viruses, Monoliths, Purification, Vaccines
PMID:
24631091
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24631091
Purification of Vero cell derived live replication deficient influenza A and B virus by ion exchange monolith chromatography.
Banjac M1, Roethl E2, Gelhart F2, Kramberger P1, Jarc BL1, Jarc M1, Strancar A1, Muster T2, Peterka M3.
Author information
Abstract
We explored the possibilities for purification of various ΔNS1 live, replication deficient influenza viruses on ion exchange methacrylate monoliths. Influenza A ΔNS1-H1N1, ΔNS1-H3N2, ΔNS1-H5N1 and ΔNS1-influenza B viruses were propagated in Vero cells and concentrated by tangential flow filtration. All four virus strains adsorbed well to CIM QA and CIM DEAE anion exchangers, with CIM QA producing higher recoveries than CIM DEAE. ΔNS1-influenza A viruses adsorbed well also to CIM SO3 cation exchanger at the same pH, while ΔNS1-influenza B virus adsorption to CIM SO3 was not complete. Dynamic binding capacity (DBC) for CIM QA, DEAE and SO3 methacrylate monoliths for influenza A ΔNS1-H1N1 virus were 2.00E+10 TCID50/ml, 1.12E+10 TCID50/ml and 9.00E+08 TCID50/ml, respectively. Purification of ΔNS1 viruses on CIM QA was scaled up and reproducibility was confirmed. Yields of infectious virus on CIM QA were between 70.8?32.3% and 87?30.8%. Total protein removal varied from 93.3?0.4% to 98.6?0.2% and host cell DNA removal efficiency was ranging from 76.4% to 99.9% and strongly depended on pretreatment with deoxyribonuclease.
Copyright ? 2014. Published by Elsevier Ltd.
KEYWORDS:
Chromatography, Influenza viruses, Monoliths, Purification, Vaccines
PMID:
24631091
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24631091