tetano
Editor, Senior Moderator
J Virol Methods. 2012 Sep 7. pii: S0166-0934(12)00304-7. doi: 10.1016/j.jviromet.2012.09.001. [Epub ahead of print]
Quantitative analysis of four rapid antigen assays for detection of pandemic H1N1 2009 compared with seasonal H1N1 and H3N2 influenza A viruses on nasopharyngeal aspirates from patients with influenza.
Chan KH, Chan KM, Ho YL, Lam YP, Tong HL, Poon LL, Cowling BJ, Peiris JS.
Source
Department of Microbiology, Queen Mary Hospital, Hong Kong. Electronic address: chankh2@hkucc.hku.hk.
Abstract
Data on analytical sensitivity of rapid diagnostic assays are important for clinical management of influenza, especially during a pandemic. Four rapid antigen detection assays were compared for detection of pandemic influenza A H1N1 2009, seasonal H1N1 and H3N2 in 96 patients with influenza A infection confirmed by real-time RT-PCR. These rapid antigen tests appeared to have lower sensitivity (55.8%) for the diagnosis of pandemic influenza A H1N1 2009 as compared with seasonal H3N2 (71.0%) or H1N1 (69.4%) influenza infections, a difference that was related to a lower viral load in patients infected with the pandemic influenza A H1N1 2009 virus. The detection limit of these antigen detection tests in clinical specimens was an influenza A M gene copy number of average 1.0?10(7)copies/ml. Significant variations between tests in sensitivity for detection of pandemic influenza A H1N1 2009 (43.4-63.3%) were observed. The Directigen EZ Influenza A+B and the Espline Influenza A+B had comparable sensitivity (63%) and were the most sensitive among the four assays evaluated.
Copyright ? 2012. Published by Elsevier B.V.
PMID:
22989408
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22989408
Quantitative analysis of four rapid antigen assays for detection of pandemic H1N1 2009 compared with seasonal H1N1 and H3N2 influenza A viruses on nasopharyngeal aspirates from patients with influenza.
Chan KH, Chan KM, Ho YL, Lam YP, Tong HL, Poon LL, Cowling BJ, Peiris JS.
Source
Department of Microbiology, Queen Mary Hospital, Hong Kong. Electronic address: chankh2@hkucc.hku.hk.
Abstract
Data on analytical sensitivity of rapid diagnostic assays are important for clinical management of influenza, especially during a pandemic. Four rapid antigen detection assays were compared for detection of pandemic influenza A H1N1 2009, seasonal H1N1 and H3N2 in 96 patients with influenza A infection confirmed by real-time RT-PCR. These rapid antigen tests appeared to have lower sensitivity (55.8%) for the diagnosis of pandemic influenza A H1N1 2009 as compared with seasonal H3N2 (71.0%) or H1N1 (69.4%) influenza infections, a difference that was related to a lower viral load in patients infected with the pandemic influenza A H1N1 2009 virus. The detection limit of these antigen detection tests in clinical specimens was an influenza A M gene copy number of average 1.0?10(7)copies/ml. Significant variations between tests in sensitivity for detection of pandemic influenza A H1N1 2009 (43.4-63.3%) were observed. The Directigen EZ Influenza A+B and the Espline Influenza A+B had comparable sensitivity (63%) and were the most sensitive among the four assays evaluated.
Copyright ? 2012. Published by Elsevier B.V.
PMID:
22989408
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22989408