tetano
Editor, Senior Moderator
J Vet Med Sci. 2017 May 1. doi: 10.1292/jvms.17-0105. [Epub ahead of print]
[h=1]Rapid diagnosis of equine influenza by highly sensitive silver amplification immunochromatography system.[/h] Yamanaka T[SUP]1[/SUP], Nemoto M[SUP]1[/SUP], Bannai H[SUP]1[/SUP], Tsujimura K[SUP]1[/SUP], Kondo T[SUP]1[/SUP], Matsumura T[SUP]1[/SUP], Fu TQH[SUP]2[/SUP], Fernandez CJ[SUP]2[/SUP], Gildea S[SUP]3[/SUP], Cullinane A[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Equine influenza (EI) is a respiratory disease caused by equine influenza A virus (EIV, H3N8) infection. Rapid diagnosis is essential to limit the disease spread. We previously reported that some rapid antigen detection (RAD) tests are fit for diagnosing EI although their sensitivity is not optimal. Here, we evaluated the performance of the newly developed RAD test using silver amplification immunochromatography (Quick Chaser Auto Flu A, B: QCA) to diagnose EI. The detection limits of QCA for EIVs were five-fold lower than the conventional RAD tests. The duration of virus antigen detection in the infected horses was longer than the conventional RAD tests. We conclude that QCA could be a valuable diagnostic method for EI.
[h=4]KEYWORDS:[/h] diagnosis; equine influenza; rapid antigen detection; silver amplification
PMID: 28458275 DOI: 10.1292/jvms.17-0105
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[h=1]Rapid diagnosis of equine influenza by highly sensitive silver amplification immunochromatography system.[/h] Yamanaka T[SUP]1[/SUP], Nemoto M[SUP]1[/SUP], Bannai H[SUP]1[/SUP], Tsujimura K[SUP]1[/SUP], Kondo T[SUP]1[/SUP], Matsumura T[SUP]1[/SUP], Fu TQH[SUP]2[/SUP], Fernandez CJ[SUP]2[/SUP], Gildea S[SUP]3[/SUP], Cullinane A[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Equine influenza (EI) is a respiratory disease caused by equine influenza A virus (EIV, H3N8) infection. Rapid diagnosis is essential to limit the disease spread. We previously reported that some rapid antigen detection (RAD) tests are fit for diagnosing EI although their sensitivity is not optimal. Here, we evaluated the performance of the newly developed RAD test using silver amplification immunochromatography (Quick Chaser Auto Flu A, B: QCA) to diagnose EI. The detection limits of QCA for EIVs were five-fold lower than the conventional RAD tests. The duration of virus antigen detection in the infected horses was longer than the conventional RAD tests. We conclude that QCA could be a valuable diagnostic method for EI.
[h=4]KEYWORDS:[/h] diagnosis; equine influenza; rapid antigen detection; silver amplification
PMID: 28458275 DOI: 10.1292/jvms.17-0105
Free full text