tetano
Editor, Senior Moderator
Sci Rep. 2017 Dec 20;7(1):17923. doi: 10.1038/s41598-017-18054-x.
[h=1]Recombinant influenza H9N2 virus with a substitution of H3 hemagglutinin transmembrane domain showed enhanced immunogenicity in mice and chicken.[/h] Zhang Y[SUP]1[/SUP], Wei Y[SUP]1[/SUP], Liu K[SUP]1[/SUP], Huang M[SUP]1[/SUP], Li R[SUP]1[/SUP], Wang Y[SUP]1[/SUP], Liu Q[SUP]1[/SUP], Zheng J[SUP]1[/SUP], Xue C[SUP]1[/SUP], Cao Y[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In recent years, avian influenza virus H9N2 undergoing antigenic drift represents a threat to poultry farming as well as public health. Current vaccines are restricted to inactivated vaccine strains and their related variants. In this study, a recombinant H9N2 (H9N2-TM) strain with a replaced H3 hemagglutinin (HA) transmembrane (TM) domain was generated. Virus assembly and viral protein composition were not affected by the transmembrane domain replacement. Further, the recombinant TM-replaced H9N2-TM virus could provide better inter-clade protection in both mice and chickens against H9N2, suggesting that the H3-TM-replacement could be considered as a strategy to develop efficient subtype-specific H9N2 influenza vaccines.
PMID: 29263359 PMCID: PMC5738434 DOI: 10.1038/s41598-017-18054-x
[h=1]Recombinant influenza H9N2 virus with a substitution of H3 hemagglutinin transmembrane domain showed enhanced immunogenicity in mice and chicken.[/h] Zhang Y[SUP]1[/SUP], Wei Y[SUP]1[/SUP], Liu K[SUP]1[/SUP], Huang M[SUP]1[/SUP], Li R[SUP]1[/SUP], Wang Y[SUP]1[/SUP], Liu Q[SUP]1[/SUP], Zheng J[SUP]1[/SUP], Xue C[SUP]1[/SUP], Cao Y[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] In recent years, avian influenza virus H9N2 undergoing antigenic drift represents a threat to poultry farming as well as public health. Current vaccines are restricted to inactivated vaccine strains and their related variants. In this study, a recombinant H9N2 (H9N2-TM) strain with a replaced H3 hemagglutinin (HA) transmembrane (TM) domain was generated. Virus assembly and viral protein composition were not affected by the transmembrane domain replacement. Further, the recombinant TM-replaced H9N2-TM virus could provide better inter-clade protection in both mice and chickens against H9N2, suggesting that the H3-TM-replacement could be considered as a strategy to develop efficient subtype-specific H9N2 influenza vaccines.
PMID: 29263359 PMCID: PMC5738434 DOI: 10.1038/s41598-017-18054-x